Figure 1
Figure 2
List of primer sets for real-time polymerase chain reaction_
| Primers | Sequences (5′<3′) | Amplicon Size |
|---|---|---|
| FGFR1-F | GAA GAC TGC TGG AGT TAA TAC C | 159 |
| FGFR1-R | TCT TCC AGG GCT TCC AGA AC | |
| FGFR3-F | CCA CTG TCT GGG TCA AGG AT | 180 |
| FGFR3-R | AGG ATG GAG CGT CTG TCA C | |
| GAPDH-F | ATC CTG GGC TAC ACT GAG C | 159 |
| GAPDH-R | CAC CAC CCT GTT GCT GTA G | |
Clinicopathological characteristics of the study subjects_
| Clinicopathological Parameters | n | % |
|---|---|---|
| Age (years): | ||
| ≤66 | 26 | 52.0 |
| >66 | 24 | 48.0 |
| Sex: | ||
| males | 43 | 86.0 |
| females | 7 | 14.0 |
| Stage: | ||
| I | 16 | 32.0 |
| II | 20 | 40.0 |
| III | 10 | 20.0 |
| IV | 4 | 8.0 |
| Grade: | ||
| low | 14 | 28.0 |
| high | 36 | 72.0 |
| Tumor type: | ||
| non invasive muscle | 15 | 30.0 |
| muscle invasive | 35 | 70.0 |
| Family history: | ||
| bladder cancer | 8 | 16.0 |
| other cancer | 6 | 12.0 |
| no cancer | 36 | 72.0 |
| Smoker: | ||
| ≤10 | 2 | 4.0 |
| >10 | 32 | 64.0 |
| non smoker | 16 | 32.0 |
| Occupational exposure | 27 | 54.0 |
Multivariate linear regression analysis of FGFR1 and FGFR3 expressions with clinicopathological variables_
| Relative Expression | Variable(s) | Undersized Coefficients B | Standard Error B | t-Test | p Value |
|---|---|---|---|---|---|
| FGFR1 | no significant variable | ||||
| FGFR3 | (constant) family history of BC cigarette smoking | –28.908 | 11.031 | –2.621 | 0.021 |
| 14.266 | 4.125 | 3.458 | 0.004 | ||
| 11.568 | 4.968 | 2.329 | 0.037 | ||