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Molecular and immunohistochemical characteristics of complete hydatidiform moles Cover

Molecular and immunohistochemical characteristics of complete hydatidiform moles

Balkan Journal of Medical Genetics
Volume 20 (2017): Issue 1 (June 2017)
By: KB Kubelka-Sabit,  I Prodanova,  D Jasar,  G Bozinovski,  V Filipovski,  S Drakulevski and  D Plaseska-Karanfilska  
Open Access
|Jun 2017

Figures & Tables

Figure 1

Microscopic appearance of the chorionic villi in complete hydatidiform mole with a pronounced stromal edema and trophoblastic proliferation (hematoxylin and eosin, ×100).
Microscopic appearance of the chorionic villi in complete hydatidiform mole with a pronounced stromal edema and trophoblastic proliferation (hematoxylin and eosin, ×100).

Figure 2

Mucoid stromal degeneration and irregular villous contours in complete hydatidiform mole (hematoxylin and eosin, ×200).
Mucoid stromal degeneration and irregular villous contours in complete hydatidiform mole (hematoxylin and eosin, ×200).

Figure 3

Ki-67 proliferation index >50.0% in villous cytotrophoblasts (×200).
Ki-67 proliferation index >50.0% in villous cytotrophoblasts (×200).

Figure 4

Absence of p57 staining In the villous cytotrophoblasts and villous stromal cells (×200).
Absence of p57 staining In the villous cytotrophoblasts and villous stromal cells (×200).

Figure 5

p63 staining in >50.0% of the villous cytotrophoblasts (×200).
p63 staining in >50.0% of the villous cytotrophoblasts (×200).

Histopathological characteristics of complete mole_

Complete MoleCharacteristicsAbsent orPresentCompleteMole Casesn = 8 (%)
Heterogenous population of villiabsentpresent0 (0.0)8 (100.0)
Circumferential trophoblastic proliferationabsentpresent1 (12.5)7 (87.5)
Cytotrophoblastic proliferationabsentpresent3 (37.5)5 (62.5)
Nuclear atypiaabsentpresent0 (0.0)8 (100.0)
Karyorrhectic stromal debrisabsentpresent6 (75 0)2 (25.0)

Immunohistochemical characteristics of complete moles_

Positive Cells (%)Ki-67Antibodyn = 8p63Antibodyn = 8p57Antibodyn = 8
n (%)n (%)n (%)
00 (0.0)0 (0.0)8 (100)
100 (0.0)0 (0.0)0 (0.0)
200 (0.0)0 (0.0)0 (0.0)
300 (0.0)0 (0.0)0 (0.0)
400 (0.0)0 (0.0)0 (0.0)
500 (0.0)0 (0.0)0 (0.0)
600 (0.0)3 (37.5)0 (0.0)
704 (50.0)2 (25.0)0 (0.0)
803 (37.5)3 (37.5)0 (0.0)
901 (12.5)0(0.0)0 (0.0)
1000 (0.0)0(0.0)0 (0.0)

Primary antibodies with clones and dilutions_

Primary Antibody (producer)CloneDilution
Monoclonal mouse anti-humanKi-67 antigen (Ventana)MIB-1RTU
Monoclonal mouse primaryantibody anti-p63 (Ventana)4A4RTU
Monoclonal mouse Primaryantibody anti-p57 (Vector Laboratories)Kip21:50

Histopathological characteristics of partial mole_

Partial Mole CharacteristicsAbsent orPresentCompleteMole Casesn = 8 (%)
Two types of villiabsentpresent8 (100.0)0(0.0)
Irregular villus contoursabsentpresent0(0.0)8 (100.0)
Trophoblastic stromal inclusionsabsentpresent7(87.5)1 (12.5)
Focal trophoblastic proliferationabsentpresent7(87.5)1 (12.5)
Presence of fetal erythrocytesabsentpresent8 (100.0)0 (0.0)
DOI: https://doi.org/10.1515/bjmg-2017-0009 | Journal eISSN: 2199-5761
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Language: English
Page range: 27 - 34
Published on: Jun 30, 2017
Published by: Macedonian Academy of Sciences and Arts
In partnership with: Paradigm Publishing Services
Publication frequency: 2 issues per year
Keywords:
Biparental diploidy,
Complete mole,
Molecular diagnostics,
p57
Related subjects:
Medicine,
Basic medical science,
Basic medical science, other

© 2017 KB Kubelka-Sabit, I Prodanova, D Jasar, G Bozinovski, V Filipovski, S Drakulevski, D Plaseska-Karanfilska, published by Macedonian Academy of Sciences and Arts
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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