Susceptibility to oral squamous cell carcinoma: correlation with variants of CYP1A1-MspI, GSTT1, GSTM1, ALDH2, EC-SOD and Lifestyle factors

Characteristics		Control Group (n = 750)	Patient Group (n = 750)	OR Value	95% CI	p Value
Sex n (%)	males females	498 (66.40%) 252 (33.60%)	504 (67.20%) 246 (32.80%)	0.97	0.78-1.20	>0.05
Age	years; mean±SD	55.51±4.42	55.49±4.33	–	–	>0.05
Smoking status n (%)	non-smoker smoker SI ≤400 SI 400	451 (60.13%) 299 (39.87%) 202 (26.93%) 97 (12.93%)	265 (35.33%) 485 (64.67%) 120 (16.00%) 365 (48.67%)	2.76 6.33	2.24-3.40 4.61-8.71	<0.01 <0.01
Drinking status n (%)	did not drink did drink alcohol DI ≤3000 DI 3000	468 (62.40%) 282 (37.60%) 172 (22.93%) 110 (14.67%)	256 (34.13%) 494 (65.87%) 114 (15.20%) 380 (50.67%)	3.20 5.21	2.59-3.96 5.79-7.16	<0.01 <0.01

OR: odds ratio; 95% CI: 95% confidence interval; SD: standard deviation; SI: smoking index; DI: drinking index.

Table 2

Distribution of CYP1A1-MspI, EC-SOD, GSTT1, GSTM1, and ALHD2 genotypes in the control and patient groups.

Genotype	Control Group		Patient Group		OR Value	95% CI	p Value
	n	%	n	%
CYP1A1-MspI: m1/m1+m1/m2 m2/m2	593 157	79.00 20.93	463 287	61.73 38.37	1.00 2.34	1.86-2.95	<0.01
EC-SOD: C/C C/G	605 145	80.67 19.33	380 370	50.67 49.27	1.00 4.06	3.23-5.12	<0.01
GSTT1: [+] [–]	598 152	79.73 20.27	395 355	52.67 47.33	1.00 3.54	2.81-43.44	<0.01
GSTM1: [+] [–]	419 331	55.87 44.13	231 519	30.80 69.20	1.00 2.83	1.95-4.47	<0.01
ALHD2: G/G non G/G	444 306	55.60 44.40	212 538	30.53 69.47	1.00 3.68	2.97-4.57	<0.01

OR: odds ratio; 95% CI: 95% confidence interval.

Table 3

The combined distribution of CYP1A1-MspI (m2/m2), EC-SOD (C/G), GSTT1[–], GSTM1 [–] and ALHD2 (non G/G) variant genotypes in the control and patient groups.

Number of Genotypes	Control Group		Patient Group		OR Value	95% CI	p Value
Number of Genotypes	n	%	n	%
0	97	12.93	8	1.07	1.00	–	<0.01
1	290	38.67	64	8.53	2.68	1.23-5.78	–
2	210	28.00	230	30.67	13.28	6.31-27.97	–
3	90	12.00	278	37.07	37.45	17.53-80.02	–
4	56	7.47	156	20.80	33.78	15.44-73.90	–
5	7	0.93	14	1.86	24.25	7.61-77.27	–

OR: odds ratio; 95% CI: 95% confidence interval.

Table 4

Combined analysis of smoking status, and variant genotype distribution in relation to oral squamous cell carcinoma susceptibility.

Number of Genotypes	Smoking Status	Control Group		Patient Group		OR Value	95% CI	p Value
Number of Genotypes	Smoking Status	n	%	n	%
0	[–]	92	12.27	31	4.13	1.00	–	–
1	[–]	118	15.73	43	5.73	1.04	0.64-1.70	0.86
2	[–]	116	15.47	90	12.00	2.22	1.44-3.44	<0.01
3	[–]	71	9.47	53	7.07	2.14	1.31-3.50	<0.01
4	[–]	40	5.33	46	6.13	3.30	1.92-5.67	<0.01
5	[–]	14	1.87	2	0.27	0.41	0.09-1.87	0.25
0	[+]	37	4.93	14	1.87	0.89	0.43-1.86	0.76
1	[+]	100	13.33	92	12.27	2.52	1.61-3.96	<0.01
2	[+]	78	10.40	147	19.60	2.43	1.65-3.58	<0.01
3	[+]	35	4.67	128	17.07	4.90	2.92-8.21	<0.01
4	[+]	44	5.87	81	10.80	1.60	0.91-2.80	0.10
5	[+]	5	0.67	23	3.07	32.20	5.49-118-91	<0.01

OR: odds ratio; 95% CI: 95% confidence interval.

Table 5

Combined analysis of smoking index, and variant genotype distribution in relation to oral squamous cell carcinoma susceptibility.

Number of Genotypes	Smoking Status	Control Group		Patient Group		OR Value	95% CI	p Value
Number of Genotypes	Smoking Status	n	%	n	%
0	≤400	45	6.00	2	0.27	1.00	–	–
1	≤400	54	7.20	16	2.13	0.22	0.05-1.00	0.07
2	≤400	45	6.00	23	3.07	0.38	0.08-1.86	0.23
3	≤400	33	4.40	44	5.87	1.00	1.00-0.21	1.00
4	≤400	23	3.07	32	4.27	1.04	0.21-5.12	0.96
5	≤400	2	0.27	3	0.40	1.12	0.11-11.60	0.92
0	400	3	0.40	4	0.53	1.82	0.51-2.85	0.49
1	400	45	6.00	24	3.20	1.80	0.85-3.80	0.12
2	400	28	3.73	123	16.40	8.60	4.50-16.44	<0.01
3	400	11	1.47	153	20.40	10.43	4.88-22.31	<0.01
4	400	9	1.20	57	7.60	4.55	1.88-11.02	<0.01
5	400	1	0.13	4	0.53	2.67	0.16-45.14	0.50

OR: odds ratio; 95% CI: 95% confidence interval.

Table 6

Combined analysis of drinking status, and variant genotype distribution in relation to oral squamous cell carcinoma susceptibility.

Number of Genotypes	Drinking Status	Control Group		Patient Group		OR Value	95% CI	p Value
Number of Genotypes	Drinking Status	n	%	n	%
0	[–]	100	13.33	27	3.60	1.00	–	–
1	[–]	166	22.13	42	5.60	0.41	0.21-0.80	0.01
2	[–]	105	14.00	77	10.27	1.18	0.61-2.28	0.62
3	[–]	51	6.80	65	8.67	2.05	1.03-4.11	0.04
4	[–]	43	5.73	43	5.73	1.61	0.78-3.32	0.20
5	[–]	3	0.40	2	0.27	1.07	0.16-7.06	0.94
0	[+]	29	3.87	18	2.40	0.44	0.21-0.90	0.03
1	[+]	52	6.93	93	12.40	7.07	4.38-11.42	<0.01
2	[+]	89	11.87	160	21.33	2.45	1.66-3.63	<0.01
3	[+]	55	7.33	116	15.47	1.66	1.02-2.69	0.04
4	[+]	41	5.47	84	11.20	2.05	1.67-3.60	0.01
5	[+]	16	2.13	23	3.07	2.16	0.32-14.41	0.43

OR: odds ratio; 95% CI: 95% confidence interval.

Table 7

Combined analysis of drinking index, and variant genotype distribution in relation to oral squamous cell carcinoma susceptibility.

Number of Genotypes	Drinking Index	Control Group		Patient Group		OR Value	95% CI	p Value
Number of Genotypes	Drinking Index	n	%	n	%
0	≤3000	21	2.80	3	0.40	1.00	–	–
1	≤3000	47	6.27	15	2.00	0.80	0.01-077	0.03
2	≤3000	49	6.53	26	3.47	0.13	0.01-1.25	0.08
3	≤3000	36	4.80	38	5.07	0.26	0.03-2.47	3.79
4	≤3000	16	2.13	28	3.73	0.44	0.05-4.26	0.48
5	≤3000	3	0.40	4	0.53	0.33	0.02-4.74	0.42
0	3000	1	0.13	4	0.53	2.96	0.55-6.28	0.08
1	3000	24	3.20	44	5.87	5.74	2.67-12.35	<0.01
2	3000	39	5.20	122	16.27	5.90	3.27-10.71	<0.01
3	3000	30	4.00	138	18.40	4.36	2.38-7.97	<0.01
4	3000	14	1.87	54	7.20	2.20	0.94-5.16	0.07
5	3000	2	0.27	18	2.40	6.75	0.83-54.66	0.07

OR: odds ratio; 95% CI: 95% confidence interval.

Table 8

Combined analysis of smoking and drinking status, and variant genotype distribution in relation to oral squamous cell carcinoma susceptibility.

Number of Genotypes	Smoking Status	Drinking Status	Controls		Patients		OR Value	95% CI	p Value
Number of Genotypes	Smoking Status	Drinking Status	n	%	n	%	OR Value
0	[–]	[–]	68	9.07	15	2.00	1.00	–	–
1	[–]	[–]	82	10.93	12	1.60	1.04	0.64-1.70	0.86
2	[–]	[–]	48	6.40	42	5.60	2.22	1.44-3.44	<0.01
3	[–]	[–]	27	3.60	21	2.80	2.14	1.31-3.50	<0.01
4	[–]	[–]	18	2.40	18	2.40	3.30	1.91-5.67	<0.01
5	[–]	[–]	1	0.13	1	0.13	0.41	0.09-1.87	0.25
0	[–]	[+]	24	3.20	16	2.13	1.91	0.31-11.81	0.48
1	[–]	[+]	36	4.80	31	4.13	7.07	4.38-11.42	<0.01
2	[–]	[+]	68	9.07	48	6.40	2.45	1.66-3.63	<0.01
3	[–]	[+]	44	5.87	32	4.27	1.66	1.02-2.69	0.04
4	[–]	[+]	22	2.93	28	3.73	2.05	1.17-3.60	0.01
5	[–]	[+]	13	1.73	1	0.13	2.16	0.32-14.40	0.43
0	[+]	[–]	32	4.27	12	1.60	0.86	0.16-4.63	0.87
1	[+]	[–]	84	11.20	30	4.00	2.53	1.51-3.96	<0.01
2	[+]	[–]	57	7.60	35	4.67	2.43	1.65-3.58	<0.01
3	[+]	[–]	24	3.20	44	5.87	4.90	2.92-8.21	<0.01
4	[+]	[–]	25	3.33	25	3.33	1.60	0.91-2.80	0.10
5	[+]	[–]	2	0.27	1	0.13	32.20	5.49-188.91	<0.01
0	[+]	[+]	5	0.67	2	0.27	3.45	1.62-5.41	<0.01
1	[+]	[+]	16	2.13	62	8.27	10.36	6.53-16.44	<0.01
2	[+]	[+]	21	2.80	112	14.93	6.20	4.12-9.33	<0.01
3	[+]	[+]	11	1.47	84	11.20	5.23	3.33-820	<0.01
4	[+]	[+]	19	2.53	56	7.47	4.19	2.67-6.58	<0.01
5	[+]	[+]	3	0.40	22	2.93	13.26	4.09-42.97	<0.01

OR: odds ratio; 95% CI: 95% confidence interval.

References

Authors

Metrics

Articles in this issue

DOI: https://doi.org/10.1515/bjmg-2016-0037 | Journal eISSN: 2199-5761 (formerly 1311-0160) | Journal ISSN: 1311-0160

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Language: English

Page range: 61 - 70

Published on: Dec 31, 2016

Published by: Macedonian Academy of Sciences and Arts

In partnership with: Paradigm Publishing Services

Keywords:

Drinking,

genetic polymorphism,

oral squamous cell carcinoma (OSCC),

smoking

Related subjects:

Medicine,

Basic medical science,

Basic medical science, other

© 2016 T-T Dong, L-J Wang, L-Z Liu, S-N Ma, published by Macedonian Academy of Sciences and Arts
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Volume 19 (2016): Issue 2 (December 2016)