Association analysis for IL13 (rs20541) in women with multiple uterine leiomyomas and controls_
| SNP ID (gene) | Genotype | Solitary ULM n=85 (%) | Multiple ULM n=96 (%) | Healthy Controls n=41 (%) | 411 Controls n=133 (%) | Statistical Analyses | Multiple ULM vs. All Controls | Multiple ULM vs. Healthy Controls | Multiple ULM vs. Normal Population | |||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CC vs. CT+TT | TT vs. CT+CC | CC vs. CT+TT | TT vs. CT+CC | CC vs. CT+TT | TT vs. CT+CC | |||||||
| rs20451 (IL13) | CC CT TT | 42(53.8) 34 (43.6) 2 (2.6) | 59(61.5) 36 (37.5) 1(1.0) | 34 (52.2) 17(39.1) 1(8.7) | 71 (53.4) 53 (39.8) 9(6.8) | ρ value OR 95% CI | 0.280 1.392 0.816-2.375 | 0.048 0.145 0.018-1.165 | 0.573 1.248 0.595-2.619 | 0.511 0.421 0.026-6.899 | 0.573 1.248 0.595-2.619 | 0.017 0.111 0.014-0.905 |
Primer sequences, primer concentrations, annealing temperatures and size of polymerase chain reaction product; restriction enzyme, enzyme concentration and size of DNA fragments after restriction for IL12B (rs6887695)_ IL12RB1 (rs11575934), 1L23R (rs7517847), 1L4 (rs2070874) and 1L4R (rs1801275)_
| SNP ID (gene) | Primer Sequence (5’-3’) | Final Concentration of Primer (nM) | Ta (°C) | PCR Product Size (bp) | Restriction Enzyme | Enzyme Concentration (units) | Allele | DNA Fragment Size After Restriction (bp) |
|---|---|---|---|---|---|---|---|---|
| rs6887695 (IL12B) | F-TTTCAGCGTGAGACCATTCA R-CCCCTAGGTCACAAGCGTAG | 250 | 55 | 245 | HphI | 0.5 | C G | 207+38 161+46+38 |
| rs11575934 (IL12RB1) | F-GGACAATTCTTACGGCCTGA R-TCTAATGCTTGCCCCTGTTC | 250 | 55 | 203 | PvuII | 0.5 | A G | 104+99 203 |
| rs7517847 (IL23R) | F-TCTGCCAATTCCCTAAAC R-AAGTAGGTGTGGATTGCC | 375 | 52 | 259 | HpyF3I | 0.25 | G τ | 259 168+91 |
| rs2070874 (IL4) | F-TAGAGATATCTTTGTCAGCATT R-AGACCCATTAATAGGTGTCG | 250 | 52 | 188 | AMI | 0.1 | C τ | 64+30 94 |
| rs1801275 (IL4R) | F-TCTCGGCCCCCACCAGTGGCGATC R-GAGGTCTTGGAAAGGCTTATAC | 125 | 60 | 418 | PvuI | 0.2 | A G | 209 186+23 |
Association analysis for IL13 (rs20541) in women with uterine leiomyomas and controls_
| SNPID (gene) | Genotype | ULM n=181 (%) | Norma] Population n=96 (%) | Healthy Controls n=41 (%) | All Controls n=133 (%) | Statistical Analyses | ULM vs. All Controls | ULM vs. Healthy Controls | ULM vs. Normal Population | |||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CC vs. CT+TT | TT vs. CT+CC | CC vs. CT+TT | TT vs. CT+CC | CC vs. CT+TT | TT vs. CT+CC | |||||||
| rs20451 (IL13) | CC CT TT | 101 (58.0) 70 (40.2) 3(1.7) | 48 (52.2) 36(39.1) 8(8.7) | 23 (52.2) 17(39.1) 1(8.7) | 71 (53.4) 53(39.8) 9(6.8) | ρ value OR 95% CI | 0.42 1.208 0.767-1.904 | 0.588 2.5 0.222-28.096 | 0.862 0.083 0.545-2.151 | 0.574 0.702 3.071-6.924 | 0.367 1.268 0.763-2.108 | 0.018 0.184 0.048-0.712 |
Logistic regression for the prediction of uterine leiomyomas subgroups_ [Dependent variable: ULM subtype (multiple vs_ solitary)_]
| Independent Variables | Exp (B) | 95% CI for Exp (B) | Coefficient ρ Value | Nag. R2 | Accuracy (%) | |
|---|---|---|---|---|---|---|
| Lower | Upper | |||||
| rs1801275 (IL4R) (G vs. Aalleles) | 3.641 | 1.206 | 10.996 | 2.19 × 10-2 | ||
| Adenomyosis (present vs. absent) | 7.967 | 2.668 | 23.790 | 2.01 × 10-4 | ||
| Age at diagnosis (years) | 1.093 | 1.036 | 1.153 | 1.17 × 10-3 | ||
| Family predisposition (negative vs. positive) | 0.200 | 0.087 | 0.462 | 1.62 × 104 | ||
| Menarche (year) | 0.445 | 0.323 | 0.613 | 7.41 × 10-7 | 0.485 | 75.0 |
| Number of pregnancies | 0.562 | 0.405 | 0.781 | 5.84 × 10-4 | ||
| Age at first sexual intercourse (year) | 0.674 | 0.533 | 0.852 | 9.59×10-4 | ||
| Constant | 7831870 | 1.40×10-6 | ||||
| rs1801275 (IL4R) (AG+GG vs. AAgenotypes) | 6.975 | 1.680 | 28.958 | 7.49 × 10-3 | ||
| Adenomyosis (present vs. absent) | 11.315 | 2.118 | 60.454 | 4.55 × 10-3 | ||
| Age at diagnosis (years) | 1.086 | 1.007 | 1.171 | 3.24 × 10-2 | ||
| Family predisposition (negative vs. positive) | 0.152 | 0.044 | 0.528 | 3.03 × 10-3 | ||
| Menarche (year) | 0.401 | 0.247 | 0.650 | 2.10×10-4 | 0.535 | 76.6 |
| Number of pregnancies | 0.559 | 0.343 | 0.912 | 1.99 × 10-2 | ||
| Age at first sexual intercourse (year) | 0.655 | 0.462 | 0.930 | 1.81 × 10-2 | ||
| Constant | 55741666 | 3.98×10-4 | ||||