Effects of nitrogen and carbon sources on the production of inulinase from strain Bacillus sp. SG113

Simeon Gavrailov; Viara Ivanova

doi:10.1515/asn-2016-0010

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Effects of nitrogen and carbon sources on the production of inulinase from strain Bacillus sp. SG113

Acta Scientifica Naturalis

Volume 3 (2016): Issue 1 (March 2016)

By: Simeon Gavrailov and Viara Ivanova

Open Access

|Mar 2016

Abstract

The effects of the carbon and nitrogen substrates on the growth of Bacillus sp. SG113 strain were studied. The use of organic nitrogen sources (peptone, beef extract, yeast extract, casein) leads to rapid cellular growth and the best results for the Bacillus strain were obtained with casein hydrolysate. From the inorganic nitrogen sources studied, the (NH₄) ₂SO₄ proved to be the best nitrogen source. Casein hydrolysate and (NH₄) ₂SO₄ stimulated the invertase synthesis. In the presence of Jerusalem artichoke, onion and garlic extracts as carbon sources the strain synthesized from 6 to 10 times more inulinase.

References

[1]. Gupta, A.K.; Kaur, N., Fructan storing plants - a potential source of high fructose syrups. J. Sci. Ind. Res. 1997, 56(8), 447-452.
Search in Google Scholar
[2]. Hendry, G., Evolutionary origins and natural functions of fructans: a climatological, biogeographic and mechanistic appraisal. New Phytol. 1993, 123(1), 3-14.
Search in Google Scholar
[3]. Vandamme, E.J.; Derycke, D.G., Microbial inulinases: Fermentation process, properties and applications. Adv. Appl. Microbiol., 1983, 29, 139-176.
Search in Google Scholar
[4]. Bajpai, P.; Margartis, A., Production of high fructose syrups from Jerusalem artichoke tubers using Kluyveromyces marxianus immobilized in agar gel. J. Gen. Appl. Microbiol., 1985, 31, 305-311.
Search in Google Scholar
[5]. Pandey, A.; Soccol, C.R.; Selvakumar, P.; Soccol, V.T.; Krieger, N.; Fontana, J.D., Recent developments in microbial inulinases, its production, properties, and industrial applications. Appl. Biochem. Biotechnol. 1999, 81(1), 35-52.
Search in Google Scholar
[6]. Neagu-Bonciu, C.; Bahrim, G., Inulinases - a versatile tool for biotechnology. Innov. Romanian Food Biotechnol. 2011, 9, 1-11.
Search in Google Scholar
[7]. Kaur, N.; Kaur, M.; Gupta, A.K.; Singh, R., Properties of β-fructosidases (invertases and inulinases) of Fusarium oxysporum grown on an aqueous extract of Cichorium intybus roots. J. Chem. Technol. Biotechnol. 1992, 53, 279-284.
Search in Google Scholar
[8]. Wei, B.; Hara, M.; Yamauchi, R.; Ueno, Y.; Kato, K., Fructooligosaccharids in the tubers of Jerusalem artichoke and Yacon. Res. Bull. Faculty Agri. Gifu Uni. 1991, 56, 133-138.
Search in Google Scholar
[9]. Chi, Z.; Zhang, T.; Liu, G.; Xue, L., Inulinase-expressing microorganisms and applications of inulinases. Appl. Microbiol. Biotechnol. 2009, 82(2), 211-220.
Search in Google Scholar
[10]. Kango, N.; Jain, S.C., Production and properties of microbial inulinases: recent advances. Food Biotechnol. 2011, 25(3), 165-212.
Search in Google Scholar
[11]. Jain, S.C.; Jain, P.C.; Kango N., Production of inulinase from Kluyveromyces marxianus using Dahlia tuber extract. Brazilian J. Microbiol. 2011, 43(1), 62-69.
Search in Google Scholar
[12]. Zittan, L., Enzymatic hydrolysis of Inulin-An alternative way to fructose production. Starch-Stärke 1981, 33(11), 373-377.10.1002/star.19810331105
Search in Google Scholar
[13]. Sangeethaa, P.T.; Ramesha, M.N.; Prapulla, S.G., Recent trends in the microbial production, analysis and application of Fructooligosaccharides. Trends Food Sci. Technol. 2005, 16, 442-457.
Search in Google Scholar
[14]. Skowronek, M.; Fiedurek, J., Inulinase biosynthesis using immobilized mycelium of Asperg illus niger. Enzyme Microb. Technol. 2006, 38, 162-167.
Search in Google Scholar
[15]. Uzunova, K.; Vassileva, A.; Kambourova, M.; Ivanova, V.; Spasova, D.; Mandeva, R.; Derekova, A.; Tonkova, A., Production and properties of a bacterial thermostable exo-inulinase. Zeitschrift für Naturforschung C 2001, 56(11-12), 1022-1028.10.1515/znc-2001-11-122011837654
Search in Google Scholar
[16]. Zherebtsov, N.A.; Shelamova, S.A.; Abramova, I.N., Biosynthesis of inulinases by Bacillus bacteria. Prikladnaya Biokhimiya i Mikrobiologiya 2002, 38(6), 634-638 (in Russian).10.1023/A:1020722510374
Search in Google Scholar
[17]. Sharma, A.D.; Kainth, S.; Gill, P.K., Inulinase production using garlic (Allium sativum) powder as a potential substrate in Streptomyces sp. J. Food Eng. 2006, 77(3), 486-491.
Search in Google Scholar
[18]. Singh, R.S.; Sooch, B.S.; Puri, M., Optimization of medium and process parameters for the production of inulinase from a newly Isolated Kluyveromyces marxianus YS-1. Biores. Technol. 2007, 98, 2518-2525.
Search in Google Scholar
[19]. Yuan, W.; Bai, F., Optimization of medium and process parameters for the production of inulinase from Kluyveromyces marxianus Y1. J. Biotechnol. 2008, 136S, S317-S318.
Search in Google Scholar
[20]. Singh, P.; Gill, P.K., Production of inulinases: recent advances. Food Technol. Biotechnol. 2006, 44 (2), 151-162.
Search in Google Scholar
[21]. Gavrailov, S.; Ivanova, V., Isolation and characteristics of a thermophilic Bacillus strain, producer of inulinase. J. BioSci. Biotechnol. 2014, SE/ONLINE, 83-94.
Search in Google Scholar
[22]. Miller, G.L, Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 1959, 3, 1426-1428.
Search in Google Scholar

Articles in this issue

DOI: https://doi.org/10.1515/asn-2016-0010 | Journal eISSN: 2603-347X | Journal ISSN: 2367-5144

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Language: English

Page range: 69 - 74

Submitted on: Oct 30, 2015

Accepted on: Mar 11, 2016

Published on: Mar 26, 2016

Published by: Konstantin Preslavski University of Shumen

In partnership with: Paradigm Publishing Services

Publication frequency: 3 issues per year

Keywords:

inulin,

Related subjects:

Life sciences, other,

Physics,

Physics, other

© 2016 Simeon Gavrailov, Viara Ivanova, published by Konstantin Preslavski University of Shumen
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Volume 3 (2016): Issue 1 (March 2016)