Abstract
Bacterial plaque has the primary etiologic role in triggering the pathological changes of periodontal disease. A major goal of periodontal therapy is supraand subgingival bacterial flora reduction through scaling and root planning, through local and general antimicrobial treatment. The aim of our study was to evaluate the effectiveness of the mechanical treatment of scaling and root planning in reducing or suppressing bacterial species from the periodontal pockets. In order to conduct this study we collected and analyzed subgingival plaque samples taken from the 50 periodontal pockets with a depth of about 5mm, from 50 subjects with diagnosis of generalized chronic periodontitis, before and after scaling and root planning. The usage of API 20A test allows a quick and easy identification of anaerobic bacteria based on biochemical properties. Additional complementary tests were used, such as examining the culture and the morpho-tinctorial features to confirm and complete the identification. The microbial flora that we were able to isolate from the periodontal pockets before scaling and root planning was very rich. After scaling and root planning the subjects showed clinical improvement in the periodontal status, and the microbiological analysis of the periodontal pockets mostly showed a quantitative and qualitative reduction of bacterial species. A local or general antimicrobial treatment is recommended to assure improved effectiveness because mechanical treatment alone cannot completely suppress bacterial flora.