Differential expression of miR-145, miR-429 and its target genes in partial reproductive tissues of swine with high and low litter size

Zhang, Xiao-Dong; Feng, Yi-Fang; Zhang, Xu; Tian, Mi; Wu, Tao; Ye, Peng-Fei; Zhang, Wei; Ding, Yue-Yun; Yin, Zong-Jun; Chu, Ming-Xing

doi:10.1515/aoas-2016-0082

Abstract

To justify the function of miRNAs in reproductive regulation in swine, the expression of miR-145, miR-429 and their related genes were studied in reproductive tissues of sows. Wannan black pig and Yorkshire pigs with extremely high (n=6) and low (n=6) litter size were sampled, and real-time quantitative PCR (qPCR) was performed on tissue samples from ovaries, uterus, oviduct, hypothalamus, and pituitary. The results indicated that miR-145, miR-429, and zinc finger E-box binding homeobox 1 gene (ZEB1) were expressed significantly different in Wannan black pig and Yorkshire pigs. In pigs with different fecundity, miR-145 in the uterus was expressed significantly lower in pigs with high litter size, than in pigs with low litter size. The miR-429 expression in the oviduct and pituitary of pigs with high litter size was significantly higher compared with tissues sampled from pigs with low litter size. The ZEB1 expression in the pituitary was lower in pigs with high litter size in comparison to pigs with low litter size, while luteinizing hormone beta subunit (LHβ) showed the opposite pattern of expression. In conclusion, miR-145 and miR-429 were differently expressed in pigs with high and low litter size and might have a role in affecting litter size of sows.

References

Bai Y., Huang J.M., Liu G., Zhang J.B., Wang J.Y., Liu C.K., Fang M.Y. (2014). Acomprehensive micro RNAexpression profile of the backfat tissue from castrated and intact full-sib pair male pigs. Bmc Genomics, 15: 1-11.
Search in Google Scholar Back to article
Chidgey K.L., Morel P.C.H., Stafford K.J., Barugh I.W. (2015). Sow and piglet productivity and sow reproductive performance in farrowing pens with temporary crating or farrowing crates on a commercial New Zealand pig farm. Livest Sci, 173: 87-94.
Search in Google Scholar Back to article
Cristobal I., Rincon R., Manso R., Carames C., Aguilera O., Madoz- Gurpide J., Rojo F., Garcia - Foncillas J. (2014). Deregulation of mi R-200b, mi R-200c and mi R-429 indicates its potential relevant role in patients with colorectal cancer liver metastasis. J. Surg. Oncol., 110: 4-5.
Search in Google Scholar Back to article
Deaver D.R., Bryan K.A. (1999). Effects of exogenous somatotropin (ST) on gonadal function in ruminants and swine. Domest. Anim. Endocrinol., 17: 287-297.
Search in Google Scholar Back to article
Donadeu F.X., Schauer S.N., Sontakke S.D. (2012). Involvement of mi RNAs in ovarian follicular and luteal development. J. Endocrinol., 215: 323-334.
Search in Google Scholar Back to article
Hasuwa H., Ueda J., Ikawa M., Okabe M. (2013). Mi R-200b and mi R-429 function in mouse ovulation and are essential for female fertility. Science, 341: 71-73.
Search in Google Scholar Back to article
Jimenez P.T., Reed B., Mendelson C.R. (2015). The mi R-200 family is hormonally regulated in mouse endometrial stromal cells and altered by superovulation. Reprod. Sci., 22: 222a-222a.
Search in Google Scholar Back to article
Lei B., Gao S., Luo L.F., Xia X.Y., Jiang S.W., Deng C.Y., Xiong Y.Z., Li F.E. (2011). A SNPin the mi R-27a gene is associated with litter size in pigs. Mol. Biol. Rep., 38: 3725-3729.
Search in Google Scholar Back to article
Li G.X., Li Y.J., Li X.J., Ning X.M., Li M.H., Yang G.S. (2011). Micro RNAidentity and abundance in developing swine adipose tissue as determined by Solexa sequencing. J. Cell. Biochem., 112: 18-28.
Search in Google Scholar Back to article
Li R., Qiao J., Wang L.N., Li L., Zhen X.M., Liu P., Zheng X.Y. (2011). Micro RNAarray and microarray evaluation of endometrial receptivity in patients with high serum progesterone levels on the day of h CGadministration. Reprod. Biol. Endocrin., 9: 29-29.
Search in Google Scholar Back to article
Li R.S., Jia Y.M., Zou H.F., Zhao R.Q. (2014). Breed-specific expression of DROSHA, DICER and AGO2 is regulated by glucocorticoid-mediated mi RNAs in the liver of newborn piglets. Anim. Genet., 45: 17-26.
Search in Google Scholar Back to article
Pillon D., Caraty A., Fabre-Nys C., Lomet D., Cateau M., Bruneau G. (2004). Regulation by estradiol of hypothalamic somatostatin gene expression: Possible involvement of somatostatin in the control of luteinizing hormone secretion in the ewe. Biol. Reprod., 71: 38-44.
Search in Google Scholar Back to article
Schauer S.N., Sontakke S.D., Watson E.D., Esteves C.L., Donadeu F.X. (2013). Involvement of mi RNAs in equine follicle development. Reproduction, 146: 273-282.
Search in Google Scholar Back to article
Sekido R., Murai K., Funahashi J., Kamachi Y., Fujisawa - Sehara A., Nabeshima Y., Kondoh H. (1994). The delta-crystallin enhancer-binding protein delta EF1 isarepressor of E2-box-mediated gene activation. Mol. Cell. Biol., 14: 5692-5700.
Search in Google Scholar Back to article
Sherman G.B., Heilman D.F., Hoss A.J., Bunick D., Lund L.A. (2001). Messenger RNAs encoding the beta subunits of guinea pig (Cavia porcellus) luteinizing hormone (gp LH) and putative chorionic gonadotropin (gp CG) are transcribed fromasingle-copy gp LH/CGbeta gene. J. Mol. Endocrinol., 26: 267-280.
Search in Google Scholar Back to article
Stephens S.M., Moley K.H. (2009). Follicular origins of modern reproductive endocrinology. Am. J. Physiol-Endoc. M., 297: E1235-E1236.
Search in Google Scholar Back to article
Tong W., Pollard J.W. (1999). Progesterone inhibits estrogen-induced cyclin D1 and cdk4 nuclear translocation, cyclin E- and cyclin A-cdk2 kinase activation, and cell proliferation in uterine epithelial cells in mice. Mol. Cell. Biol., 19: 2251-2264.
Search in Google Scholar Back to article
van Rensburg L.J., Spencer B.T. (2014). The influence of environmental temperatures on farrowing rates and litter sizes in South African pig breeding units. Onderstepoort J. Vet. Res., 81: 1-7.
Search in Google Scholar Back to article
Wang Z., Chen Q., Yang Y., Yang H., He P., Zhang Z., Chen Z., Liao R., Tu Y., Zhang X., Wang Q., Pan Y. (2014). Agenome-wide scan for selection signatures in Yorkshire and Landrace pigs based on sequencing data. Anim. Genet., 45: 808-816.
Search in Google Scholar Back to article
Wienholds E., Plasterk R.H.A. (2005). Micro RNAfunction in animal development. Febs Lett., 579: 5911-5922.
Search in Google Scholar Back to article
Woo I., Tobler K., Khafagy A., Christianson M.S., Yates M., Garcia J. (2015). Predictive value of elevated LH/FSHratio for ovulation induction in patients with polycystic ovary syndrome. J. Reprod. Med., 60: 495-500.
Search in Google Scholar Back to article
Yan G.J., Zhang L.X., Fang T., Zhang Q., Wu S.G., Jiang Y., Sun H.X., Hu Y.L. (2012). Micro RNA-145 suppresses mouse granulosa cell proliferation by targeting activin receptor IB. Febs Lett., 586: 63-70.
Search in Google Scholar Back to article
Ye Z.B., Ma G., Zhao Y.H., Xiao Y., Zhan Y., Jing C., Gao K., Liu Z.H., Yu S.J. (2015). mi R-429 inhibits migration and invasion of breast cancer cells in vitro. Int. J. Oncol., 46: 1-8.
Search in Google Scholar Back to article
Yuan D.Z., Yu L.L., Qu T., Zhang S.M., Zhao Y.B., Pan J.L., Xu Q., He Y.P., Zhang J.H., Yue L.M. (2015). Identification and characterization of progesterone- and estrogen-regulated micro RNAs in mouse endometrial epithelial cells. Reprod. Sci., 22: 23-34.
Search in Google Scholar Back to article

Differential expression of miR-145, miR-429 and its target genes in partial reproductive tissues of swine with high and low litter size

Abstract

Paradigm

My account