Broiler health status has a major negative impact on broiler flock contamination with Campylobacter spp. in Lithuania

Lėgaudaitė-Lydekaitienė, Viktorija; Šernienė, Loreta; Vismantaitė, Vaida; Malakauskas, Mindaugas; Kudirkienė, Eglė

doi:10.1515/aoas-2016-0080

Abstract

The objective of this study was to determine risk factors for Campylobacter infection in broiler flocks in Lithuania. Each broiler flock was tested for the contamination with Campylobacter spp., and various broiler farm, flock and abattoir as well as the weather-associated characteristics were analysed using the statistical package SPSS. Study revealed that 59.3% of the examined broiler flocks were contaminated with Campylobacter spp. Statistical analysis revealed that broiler flock contamination with Campylobacter was abattoir- and farm-dependent. Among a number of risk factors (e.g. the number of broiler houses at the farm, the type of ventilation system, the presence of the anteroom and boot security, etc.) identified, two broiler health-associated characteristics: (i) broiler age and (ii) the average weight per bird at abattoir had the highest impact on the increased prevalence of Campylobacter spp. in broilers. According to our results broiler health status has a major negative effect on broiler flock contamination with Campylobacter. Thus, it needs to be considered when improving control of Campylobacter spp. in broilers.

References

Allain V., Chemaly M., Laisney M.J., Rouxel S., Quesne S., Le Bouquin S. (2014). Prevalence of and risk factors for Campylobacter colonisation in broiler flocks at the end of the rearing period in France. Brit. Poultry Sci., 55: 452-459.
Search in Google Scholar Back to article
Allen V.M., Bull S.A., Corry J.E.L., Domingue G., Jorgensen F., Frost J.A., White R., Gonzalez A., Elvis N., Humphrey T.J. (2007). Campylobacter spp. contamination of chicken carcasses during processing in relation to flock colonization. Int. J. Food Microbiol., 113: 54-61.
Search in Google Scholar Back to article
Alves L., Voss - Rech D., Santiago Silvia V., Dos Santos Pozza J., Gasparet to A., Luiz Vaz C.S. (2012). Study of thermophilic Campylobacter contamination of broiler batch at slaughter. Acta Sci. Vet., 40: 1-8.
Search in Google Scholar Back to article
Ansari - Lari M., Hosseinzadeh S., Shahram Shekarforoush S., Abdollahi M., Berizi E. (2011). Prevalence and risk factors associated with campylobacter infections in broiler flocks in Shiraz, southern Iran. Int. J. Food Microbiol., 144: 475-479.
Search in Google Scholar Back to article
Arsenault J., Letellier A., Quessy S., Normand V., Boulianne M. (2007). Prevalence and risk factors for Salmonella spp. and Campylobacter spp. caecal colonization in broiler chicken and turkey flocks slaughtered in Quebec, Canada. Prev. Vet. Med., 81: 250-264.
Search in Google Scholar Back to article
Barrios P.R., Reiersen J., Lowman R., Bisaillon J.R., Michel P., Fridriksdottir V., Gunnarsson E., Stern N., Berke O., Mc Ewen S., Martin W. (2006). Risk factors for Campylobacter spp. colonization in broiler flocks in Iceland. Prev. Vet. Med., 74: 264-278.
Search in Google Scholar Back to article
BC Poultry Association (2006). BC Poultry Biosecurity Reference Guide, B. Cox (ed.). Available on: http://bcchicken.ca/wp-content/uploads/2012/11/FullCopyUpdatedJan2012.pdf
Search in Google Scholar Back to article
Bouwknegt M., Dam-Deisz W.D.C., Schouten J.M., Wannet W.J.B., Van Pelt W., Visser G., Vande Giessen A.W. (2003). Surveillance of zoonotic bacteria in farm animals in The Netherlands. RIVM-National Institute of Public Health and the Environment, Bilthoven, The Netherlands, Report ID 285859013. Available on: http://www.rivm.nl/bibliotheek/rapporten/285859013.html.
Search in Google Scholar Back to article
Bouwknegt M., Vande Giessen A.W., Dam-Deisz W.D.C., Havelaar A.H., Nagelkerke N.J.D., Henken A.M. (2004). Risk factors for the presence of Campylobacter spp. in Dutch broiler flocks. Prev. Vet. Med., 62: 35-49.
Search in Google Scholar Back to article
Bull S.A., Allen V.M., Domingue G., Jorgensen F., Frost J.A., Ure R., Whyte R., Tinker D., Corry J.E.L., Gillard-King J., Humphrey T.J. (2006). Sources of Campylobacter spp. colonizing housed broiler flocks during rearing. Appl. Environ. Microbiol., 72: 645-652.
Search in Google Scholar Back to article
Bull S.A., Thoma A., Humphrey T., Ellis- Iversen J., Cook A.J., Lovell R., Jorgensen F. (2008). Flock health indicators and Campylobacter spp. in commercial housed broilers reared in Great Britain. Appl. Environ. Microbiol., 74: 5408-5413.
Search in Google Scholar Back to article
Chowdhury S., Sandberg M., Themudo G.E., Ersboll A.K. (2012). Risk factors for Campylobacter infection in Danish broiler chickens. Poultry Sci., 91: 2701-2709.
Search in Google Scholar Back to article
Comin D., Valero A., Manfreda G., Garcia - Gimeno R.M., Paiusco A., De Medici D., Terza P., Ferrarini S., De Cesare A. (2014). Microbiological criteria for Campylobacter in broiler carcasses in Italy: Apossible approach to derive them. Int. J. Food Microbiol., 184: 64-68.
Search in Google Scholar Back to article
Ellis- Iversen J., Jorgensen F., Bull S., Powell L., Cook A.J., Humphrey T.J. (2009). Risk factors for Campylobacter colonization during rearing of broiler flocks in Great Britain. Prev. Vet. Med., 89: 178-184.
Search in Google Scholar Back to article
European Food Safety Authority(2014). The European Union Summary Report on Trends and Sources of Zoonoses, Zoonotic Agents and Food-borne Outbreaks in 2012. EFSA Journal, 12: 1-312.
Search in Google Scholar Back to article
European Food Safety Authority(2015). The European Union Summary Report on Trends and Sources of Zoonoses, Zoonotic Agents and Food-borne Outbreaks in 2014. EFSA Journal, 13: 1-91.
Search in Google Scholar Back to article
Evans S.J., Sayers A.R. (2000). Alongitudinal study of Campylobacter infection of broiler flocks in Great Britain. Prev. Vet. Med., 46: 209-223.
Search in Google Scholar Back to article
Frediani W., Stephan R. (2003). Resistance patterns of Campylobacter spp. strains isolated from poultry carcasses inabig Swiss poultry slaughterhouse. Int. J. Food Microbiol., 89: 233-240.
Search in Google Scholar Back to article
Gibbens J.C., Pascoe S.J.S., Evans S.J., Davies R.H., Sayers A.R. (2001). Atrial of biosecurity asameans to control Campylobacter infection of broiler chickens. Prev. Vet. Med., 48: 85-99.
Search in Google Scholar Back to article
Guerin M.T., Martin W., Reierse J., Berke O., Mc Ewen S.A., Bisaillon J.R., Lowman R. (2007). Afarm-level study of risk factors associated with the colonization of broiler flocks with Campylobacter spp. in Iceland, 2001-2004. Acta Vet. Scand., 49: 1-12.
Search in Google Scholar Back to article
Guerin M.T., Martin W., Reierse J., Berke O., Mc Ewen S.A., Bisaillon J.R., Lowman R. (2007). House-level risk factors associated with the colonization of broiler flocks with Campylobacter spp. in Iceland, 2001-2004. Acta Vet. Scand., 3: 1-12.
Search in Google Scholar Back to article
Hald B., Wedderkopp A., Madsen M. (2000). Thermophilic Campylobacter spp. in Danish broiler production: Across-sectional survey andaretrospective analysis of risk factors for occurrence in broiler flocks. Avian Pathol., 29: 123-131.
Search in Google Scholar Back to article
Hald B., Skovgard H., Pedersen K., Bunkenborg H. (2008). Influxed insects as vectors for Campylobacter jejuni and Campylobacter coli in Danish broiler houses. Poultry Sci., 74: 5408-5413.
Search in Google Scholar Back to article
Hanning I., Jarquin R., Slavik M. (2008). Campylobacter jejuni asasecondary colonizer of poultry biofilms. J. Appl. Microbiol., 105: 1199-1208.
Search in Google Scholar Back to article
Hansson I., Forshell L.P., Gustafsson P., Boqvist S., Lindblad J., Engvall E.O., Andersson Y., Vågsholm I. (2007). Summary of the Swedish Campylobacter program in broilers, 2001 through 2005. J. Food Protect., 70: 2008-2014.
Search in Google Scholar Back to article
Henry I., Reichardt J., Denis M., Cardinale E. (2011). Prevalence and risk factors for Campylobacter spp. in chicken broiler flocks in Reunion Island (Indian Ocean). Prev. Vet. Med., 100: 64-70.
Search in Google Scholar Back to article
Hofshagen M., Kruse H. (2005). Reduction in flock prevalence of Campylobacter spp. in broilers in Norway after implementation of an action plan. J. Food Protect., 68: 2220-2223.
Search in Google Scholar Back to article
Hue O., Le Bouquin S., Laisney M., Allain V., Lalande F., Petetin I., Rouxel S., Quesne S., Gloaguen P., Picherot M., Santolini J., Salvat G., Bougeard S., Chemaly M. (2010). Prevalence of and risk factors for Campylobacter spp. contamination of broiler chicken carcasses at the slaughterhouse. Food Microbiol., 27: 992-999.
Search in Google Scholar Back to article
Hughes R.A., Hallett K., Cogan T., Enser M., Humphrey T. (2009). The Response of Campylobacter jejuni to low temperature differs from that of Escherichia coli. Appl. Environ. Microbiol., 75: 6292-6298.
Search in Google Scholar Back to article
Humphrey T.J. (2006). Are happy chickens safer chickens? Poultry welfare and disease susceptibility. Brit. Poultry Sci., 47: 379-391.
Search in Google Scholar Back to article
Huneau - Salaun A., Denis M., Balaine L., Salvat G. (2007). Risk factors for Campylobacter spp. colonization in French free-range broiler-chicken flocks at the end of the indoor rearing period. Prev. Vet. Med., 80: 34-48.
Search in Google Scholar Back to article
Ica T., Caner V., Istanbullu O., Nguyen H.D., Ahmed B., Call D.R., Beyenal H. (2012). Characterization of mono- and mixed-culture Campylobacter jejuni biofilms. Appl. Environ. Microbiol., 78: 1033-1038.
Search in Google Scholar Back to article
Kapperud G., Skjerve E., Vik L., Hauge K., Lysaker A., Aalmen I., Ostroff S.M., Potter M. (1993). Epidemiological investigation of risk factors for Campylobacter colonization in Norwegian broiler flocks. Epidemiol. Infect., 111: 245-255.
Search in Google Scholar Back to article
Katsma W.E., De Koeijer A.A., Jacobs - Reitsma W.F., Mangen M.J., Wagenaar J.A. (2007). Assessing interventions to reduce the risk of Campylobacter prevalence in broilers. Risk Anal., 27: 863-876.
Search in Google Scholar Back to article
Katzav M., Isohanni P., Lund M., Hakkinen M., Lyhs U. (2008). PCRassay for the detection of Campylobacter in marinated and non-marinated poultry products. Food Microbiol., 25: 908-914.
Search in Google Scholar Back to article
Kudirkienė E., Malakauskas M., Malakauskas A., Bojesen A.M., Olsen J.E. (2010). Demonstration of persistent strains of Campylobacter jejuni within broiler farms over a 1-year period in Lithuania. J. Appl. Microbiol., 108: 868-877.
Search in Google Scholar Back to article
Lawes J.R., Vidal A., Clifton- Hadley F.A., Sayers R., Rodgers J., Snow L., Evans S.J., Powell L.F. (2012). Investigation of prevalence and risk factors for Campylobacter in broiler flocks at slaughter: results froma UKsurvey. Epidemiol. Infect., 140: 1725-1737.
Search in Google Scholar Back to article
Lyngstad T.M., Jonsson M.E., Hofshagen M., Heier B.T. (2008). Risk factors associated with the presence of Campylobacter species in Norwegian broiler flocks. Poultry Sci., 87: 1987-1994.
Search in Google Scholar Back to article
Mc Dowell S.W., Menzies F.D., Mc Bride S.H., Oza A.N., Mc Kenna J.P., Gordon A.W., Neill S.D. (2008). Campylobacter spp. in conventional broiler flocks in Northern Ireland: epidemiology and risk factors. Prev. Vet. Med., 84: 261-276.
Search in Google Scholar Back to article
Messad S., Hamdi T.M., Bouhamed R., Ramdani- Bouguessa N., Tazir M. (2014). Frequency of contamination and antimicrobial resistance of thermotolerant Campylobacter isolated from some broiler farms and slaughterhouses in the region of Algiers. Food Control, 40: 324-328.
Search in Google Scholar Back to article
Nather G., Alter T., Martin A., Ellerbroek L. (2009). Analysis of risk factors for Campylobacter species infection in broilers flocks. Poultry Sci., 88: 1299-1305.
Search in Google Scholar Back to article
Newell D.G., Fearnley C. (2003). Sources of Campylobacter colonization in broiler chickens. Appl. Environ. Microbiol., 69: 4343-4351.
Search in Google Scholar Back to article
Newell D.G., Allen V., Elvers K., Dorfper D., Hanssen I., Jones P., James S., Gittins J., Stern N., Davies R., Pearson D., Salvat G. (2014). Acritical review of interventions and strategies (both biosecurity and non-biosecurity) to reduce Campylobacter on the poultry farm. Veterinary Laboratories Agency, UK, Report ID B15025, available on: http://www.foodbase.org.uk/results.php?f_report_id=384.
Search in Google Scholar Back to article
Posch J., Feierl G., Wuest G., Sixl W., Schmidt S., Haas D.U., Reinthaler F.F., Marth E. (2006). Transmission of Campylobacter spp. inapoultry slaughterhouse and genetic characterisation of the isolates by pulsed-field gel electrophoresis. Brit. Poultry Sci., 47: 286-293.
Search in Google Scholar Back to article
Ramabu S.S., Boxall N.S., Madie P., Fenwick S.G. (2004). Some potential sources for transmission of Campylobacter jejuni to broiler chickens. Lett. Appl. Microbiol., 39: 252-256.
Search in Google Scholar Back to article
Ramonaitė S., Kudirkienė E., Tamulevičienė E., Levinienė G., Malakauskas A., Golz G., Alter T., Malakauskas M. (2014). Prevalence and genotypes of Campylobacter jejuni from urban environmental sources in comparison with clinical isolates from children. J. Med. Microbiol., 63: 1205-1213.
Search in Google Scholar Back to article
Refregier- Petton J., Rose N., Denis M., Salvat G. (2001). Risk factors for Campylobacter spp. contamination in French broiler-chicken flocks at the end of the rearing period. Prev. Vet. Med., 50: 89-100.
Search in Google Scholar Back to article
Rodrigues L.C., Cowden J.M., Wheeler J.G., Sethi D., Wall P.G., Cumberland P., Tompkins D.S., Hudson M.J., Roberts J.A., Roderick P.J. (2001). The study of infectious intestinal disease in England risk factors for cases of infectious intestinal disease with Campylobacter jejuni infection. Epidemiol. Infect., 127: 185-193.
Search in Google Scholar Back to article
Rosenquist H., Sommer H.M., Nielsen N.L., Christensen B.B. (2006). The effect of slaughter operations on the contamination of chicken carcasses with thermotolerant Campylobacter. Int. J. Food Microbiol., 108: 226-232.
Search in Google Scholar Back to article
Rosenquist H., Boysen L., Galliano C., Nordentoft S., Ethelberg S., Borck B. (2009). Danish strategies to control Campylobacter in broilers and broiler meat: Facts and effects. Epidemiol. Infect., 137: 1742-1750.
Search in Google Scholar Back to article
Sanders S.Q., Boothe D.H., Frank J.F., Arnold J.W. (2007). Culture and detection of Campylobacter jejuni within mixed microbial populations of biofilms on stainless steel. J. Food Protect., 70: 1379-1385.
Search in Google Scholar Back to article
Silva J., Leite D., Fernandes M., Mena C., Gibbs P.A., Teixeira P. (2011). Campylobacter spp. asafood borne pathogen:areview. Front Microbiol., 2: 1-12.
Search in Google Scholar Back to article
Stern N.J., Fedorka- Cray P., Bailey J.S., Cox N.A., Craven S.F., Hiet K.L., Musgrove M.T., Ladely S., Cosby D., Mead G.C. (2001). Distribution of Campylobacter spp. in selected U.S. poultry production and processing operations. J. Food Protect., 64: 1705-1710.
Search in Google Scholar Back to article
Tam C.C., Higgins C.D., Neal K.R., Rodrigues L.C., Millership S.E., O ’ Brien S.J., and on behalf of the Campylobacter Case-Control Study Group (2009). Chicken consumption and use of acid-suppressing medications as risk factors for Campylobacter enteritis, England. Emerg. Infect. Dis. J., 15: 1402-1408.
Search in Google Scholar Back to article
Trachoo N., Frank J.F., Stern N.J. (2002). Survival of Campylobacter jejuni in biofilms isolated from chicken houses. J. Food Protect., 65: 1110-1116.
Search in Google Scholar Back to article
Vandeplas S., Marq C., Dubois Dauphin R., Beckers Y., Thonart P., Thewis A. (2008). Contamination of poultry flocks by the human pathogen Campylobacter spp. and strategies to reduce its prevalence at the farm level. Biotechnol. Agron. Soc. Environ., 12: 317-334.
Search in Google Scholar Back to article
Vashin I.T., Stoyanchev T.T. (2011). Influence of temperature on Campylobacter jejuni survival rates in pork meat. Bulg. J. Vet. Med., 14: 25−30.
Search in Google Scholar Back to article
Wang R.F., Slavik M.F., Cao W.W. (1992). Arapid PCRmethod for direct detection of low numbers of Campylobacter jejuni. J. Rapid Meth. Aut. Mic., 1: 101-108.
Search in Google Scholar Back to article
Williams J.K., Sait L.C., Cogan T.A., Jorgensen F., Grogono- Thomas R., Humphrey T.J. (2012). Enrichment culture can bias the isolation of Campylobacter subtypes. Epidemiol. Infect., 140: 1227-1235.
Search in Google Scholar Back to article
Wingstrand A., Neimann J., Engberg J., Nielsen E.M., Gerner - Smidt P., Wegener H.C., Molbak K. (2006). Fresh chicken as main risk factor for campylobacteriosis, Denmark. Emerg. Infect. Dis., 12: 280-284.
Search in Google Scholar Back to article

Broiler health status has a major negative impact on broiler flock contamination with Campylobacter spp. in Lithuania

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