Effect of β-lactoglobulin gene polymorphism, lactation stage and breed on milk traits in Chios and Karagouniko sheep breeds

Triantaphyllopoulos, Kostas A.; Koutsouli, Panagiota; Kandris, Athanassios; Papachristou, Dimitris; Markopoulou, Kalliopi E.; Mataragka, Antonia; Massouras, Theofylaktos; Bizelis, Iosif

doi:10.1515/aoas-2016-0058

Abstract

The animal selection with favourable phenotypes of the past has been, currently, replaced by the genotype selection on quantitative traits, assisted by the expanding molecular techniques in the context of livestock improvement. In this study, the c.112T>C polymorphism in exon II of β-lactoglobulin (β-LG) gene was investigated in Karagouniko and Chios sheep breeds by using polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP), and possible associations with milk traits were examined. In total, 125 blood DNA samples were isolated for PCR-RFLP analysis and the respective 217 milk samples′ composition profile was obtained. The goodness of fit test to Hardy-Weinberg equilibrium (HWE) for β-LG genotypes was estimated and associations found between β-LG genotypes and raw milk composition. Two alleles and three genotypes were observed (AA, AB and BB) in both breeds, and Chios breed significantly deviated (P≤0.05) from Hardy-Weinberg equilibrium (HWE). Conclusively, linear mixed model analysis on samples, from both breeds collectively, showed significant effects of β-LG genotype on lactose percentage and somatic cell count (SCC), lactation stage on daily milk yield and protein, while the breed effect was significant only on daily milk yield.

References

Amigo L., Recio I., Ramos M. (2000). Genetic polymorphism of ovine milk proteins. Int. Dairy J., 10: 135-149.
Search in Google Scholar Back to article
Arora R., Bhatia S., Mishra B.P., Sharma R., Pandey A.K., Prakash B., Jain A. (2010). Genetic polymorphism of the beta-lactoglobulin gene in native sheep from India. Biochem. Genet., 48: 304-311.
Search in Google Scholar Back to article
Aschaffenburg R., Drewry J. (1955). Occurrence of different beta-lactoglobulins in cow’s milk. Nature, 176: 218-219.
Search in Google Scholar Back to article
Bionaz M., Loor J.J. (2008). ACSL1, AGPAT6, FABP3, LPIN1, and SLC27A6 are the most abundant isoforms in bovine mammary tissue and their expression is affected by stage of lactation. J. Nutr., 138: 1019-1024.
Search in Google Scholar Back to article
Corral J.M., Padilla J.A., Izquierdo M. (2010). Associations between milk protein genetic polymorphisms and milk production traits in Merino sheep breed. Livest. Sci., 129: 73-79.
Search in Google Scholar Back to article
Dario C., Carnicella D., Dario M., Bufano G. (2008). Genetic polymorphism of β-lactoglobulin gene and effect on milk composition in Leccese sheep. Small Rumin. Res., 74: 270-273.
Search in Google Scholar Back to article
Erhardt G. (1989). Evidence forathird allele at the β-lactoglobulin (β-Lg) locus of sheep milk and its occurrence in different breeds. Anim. Genet., 20: 197-204.
Search in Google Scholar Back to article
Feligini M., Parma P., Aleandri R., Greppi G.F., Enne G. (1998). PCR-RFLPtest for direct determination of beta-lactoglobulin genotype in sheep. Anim. Genet., 29: 473-474.
Search in Google Scholar Back to article
Gaye P., Hue-Delahaie D., Mercier J.C., Soulier S., Vilotte J.L., Furet J.P. (1986). Ovine beta-lactoglobulin messenger RNA: nucleotide sequence and m RNAlevels during functional differentiation of the mammary gland. Biochimie, 68: 1097-1107.
Search in Google Scholar Back to article
Heaton M.P., Leymaster K.A., Kalbfleisch T.S., Kijas J.W., Clarke S.M., Mc - Ewan J., Maddox J.F., Basnayake V., Petrik D.T., Simpson B., Smith T.P., Chitko-McKown C.G. (2014). SNPs for parentage testing and traceability in globally diverse breeds of sheep. PLo S One, 9(4):e94851.
Search in Google Scholar Back to article
Hejtmánková A., Pivec V., Trnková E., Dragounová H. (2012). Differences in the composition of total and whey proteins in goat and ewe milk and their changes throughout the lactation period. Czech J. Anim. Sci., 57: 323-331.
Search in Google Scholar Back to article
Johnson M.E., Chen C.M., Jaeggi J.J. (2001). Effect of rennet coagulation time on composition, yield, and quality of reduced-fat cheddar cheese. J. Dairy Sci., 84: 1027-1033.
Search in Google Scholar Back to article
Kawecka A., Radko A. (2011). Genetic polymorphism of β-lactoglobulin in sheep raised for milk production. J. Appl. Anim. Res., 39: 68-71.
Search in Google Scholar Back to article
Kolde H.J., Braunitzer G. (1983). The primary structure of ovine beta-lactoglobulin, 2: Discussion and genetic aspects. Milchwissenschaft, 38: 70-71.
Search in Google Scholar Back to article
Kuchtík J., Šustová K., Urban T., Zapletal D. (2008). Effect of the stage of lactation on milk composition, its properties and the quality of rennet curdling in East Friesian ewes. Czech J. Anim. Sci., 53: 55-63.
Search in Google Scholar Back to article
Kusza S., Sziszkosz N., Nagy K., Masala A., Kukovics S., András J. (2015). Preliminary result ofagenetic polymorphism of β-lactoglobulin gene and the phylogenetic study of ten Balkan and Central European indigenous sheep. Acta Biochim. Pol,, 62: 109-112.
Search in Google Scholar Back to article
Mastrangelo S., Di Gerlando R., Tolone M., Tortorici L., Sardina M.T., Portolano B., International Sheep Genomics Consortium (2014). Genome wide linkage disequilibrium and genetic structure in Sicilian dairy sheep breeds. BMC Genet., 15: 108.
Search in Google Scholar Back to article
McKusick C.B., Thomas L.D., Berger M.Y. (2001). Effect of weaning system on commercial milk production and lamb growth of East Friesian dairy sheep. J. Dairy Sci., 84: 1660-1668.
Search in Google Scholar Back to article
Mierlita D. (2011). Effects of breed on milk fatty acid profile in dairy ewes, with particular reference to cis-9, trans-11 conjugated linoleic acid. S. Afr. J. Anim. Sci., 41: 223-223.
Search in Google Scholar Back to article
Miller S.A., Dykes D.D., Polesky H.F. (1988). Asimple salting out procedure for extracting DNAfrom human nucleated cells. Nucleic Acids Res., 16: 1215.
Search in Google Scholar Back to article
Moatsou G., Hatzinaki A., Samolada M., Anifantakis E. (2005). Major whey proteins in ovine and goat acid wheys from indigenous Greek breeds. Int. Dairy J., 15: 123-131.
Search in Google Scholar Back to article
Orancova M., Margetín M., Tančin V. (2015). The effect of stage of lactation on daily milk yield, and milk fat and protein content in Tsigai and Improved Valachian ewes. Mljekarstvo, 65: 48-56.
Search in Google Scholar Back to article
Recio I., Fernandez-Fournier A., Martin - Alvarez P.J., Ramos M. (1997). ß-lactoglobulin polymorphism in ovine breeds: influence on cheesemaking properties and milk composition. Lait, 77: 259-265.
Search in Google Scholar Back to article
Rozbicka - Wieczorek A., Radzik - Rant A., Rant W., Puppel K. (2015). The effect of breed, ß-lactoglobulin variants and cell count on yield, chemical components and whey protein composition in milk of non-dairy sheep. J. Anim. Plant Sci., 25: 633-639.
Search in Google Scholar Back to article
Selvaggi M., Laudadio V., Dario C., Tufarelli V. (2014). Investigating the genetic polymorphism of sheep milk proteins:auseful tool for dairy production. J. Sci. Food Agr., 94: 3090-3099.
Search in Google Scholar Back to article
Selvaggi M., Laudadio V., Dario C., Tufarelli V. (2015). β-lactoglobulin gene polymorphisms in sheep and effects on milk production traits: Areview. Adv. Anim. Vet. Sci., 3: 478-484.
Search in Google Scholar Back to article
Wu Y., Fan H., Wang Y., Zhang L., Gao X., Chen Y., Li J., Ren H., Gao H. (2014). Genome- wide association studies using haplotypes and individual SNPs in Simmental cattle. PLo S One, 9(10):e109330.
Search in Google Scholar Back to article
Yousefi S., Ahani Azari M., Zerehdaran S., Samiee R., Khataminejhad R. (2013). Effect of β-lactoglobulin and κ-casein genes polymorphism on milk composition in indigenous Zel sheep. Arch. Tierzucht, 21: 216-224.
Search in Google Scholar Back to article

Effect of β-lactoglobulin gene polymorphism, lactation stage and breed on milk traits in Chios and Karagouniko sheep breeds

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