Hyperglycemia-induced changes in resistin gene expression in white adipose tissue in piglets

Ocłoń, Ewa; Zubel-Łojek, Joanna; Latacz, Anna; Pierzchała-Koziec, Krystyna

doi:10.1515/aoas-2015-0021

Abstract

Previous data strongly indicated that resistin, an adipocyte-derived signalling peptide, plays an important role in metabolism and glucose homeostasis. Thus, the aim of the present study was to examine changes in synthesis and concentration of resistin in white adipose tissue in response to hyperglycemia in piglets. In order to develop hyperglycemia, piglets (10-week-old, Polish Landrace fatteners, female) received intraperitoneal (ip) injection of 150 mg streptozotocin (HI, n=6) or 60 mg synthetic glucocorticoid (HII, n=6). An injection of NaCl physiological saline was used as a control (n=6). Plasma resistin level was significantly higher in HII group compared with the control, while no difference was observed in HI. In epicardial adipose tissue (EAT ) the resistin mRNA level significantly increased whereas the opposite effect was observed for omental fat tissue (OAT ) in both experimental groups. Additionally, the resistin concentration did not change in EAT ; however, it was decreased in omental adipose tissue in response to hyperglycemia. The results obtained indicate that activity of resistin strongly depends on glycemic status as well as adipose tissue localization.

References

Aldhahi W., Hamdy O. (2003). Adipokines, inflammation, and the endothelium in diabetes. Curr. Diab. Rep., 3: 293-298.
Search in Google Scholar
Antuna - Puente B., Feve B., Fellahi S., Bastard J.P. (2008). Adipokines: the missing link between insulin resistance and obesity. Diabetes Metab., 34: 2-11.
Search in Google Scholar
Baker A.R., Silva D.W., Quinn A.L., Harte D., Pagano R.S., Bonser S., Kumar P.G., Mc Ternan N.H. (2006). Human epicardial adipose tissue expressesapathogenic profile of adipocytokines in patients with cardiovascular disease. Cardiovasc. Diabetol., 13: 5-10.
Search in Google Scholar
Bakker W., Eringa E.C., Sipkema P.,van Hinsbergh V.W. (2009). Endothelial dysfunction and diabetes: roles of hyperglycemia, impaired insulin signaling and obesity. Cell Tissue Res., 335: 165-189.
Search in Google Scholar
Banerjee R.R., Rangwala S.M., Shapiro J.S., Rich A.S., Rhoades B., Qi Y., Wang J., Rajala M.W., Pocai A., Scherer P.E., Steppan C.M., Ahima R.S., Obici S., Rossetti L., Lazar M.A. (2004). Regulation of fasted blood glucose by resistin. Science, 303: 1195-1198.
Search in Google Scholar
Brown J.E., Onyango D.J., Dunmore S.J. (2007). Resistin down-regulates insulin receptor expression, and modulates cell viability in rodent pancreatic beta-cells. FEBS Lett., 581: 3273-3276.
Search in Google Scholar
Chu S.W., Ding K., Li Y., Pang C., Tang C. (2008). Plasma resistin associated with myocardium injury in patients with acute coronary syndrome. Circ. J., 72: 1249-1253.
Search in Google Scholar
Dai M.H., Xia T., Chen X.D., Gan L., Feng S.Q., Qiu H., Peng Y., Yang Z.Q. (2006). Cloning and characterization of porcine resistin gene. Domest. Anim. Endocrinol., 30: 88-97.
Search in Google Scholar
Fantuzzi G. (2005). Adipose tissue, adipokines, and inflammation. J. Allergy Clin. Immunol., 115: 911-919.
Search in Google Scholar
Fu Y., Luo L., Luo N., Garvey W.T. (2006). Proinflammatory cytokine production and insulin sensitivity regulated by overexpression of resistin in 3T3-L1 adipocytes. Nutr. Metab. (Lond)., 19: 3-28.
Search in Google Scholar
Gao J., Chang Chua C., Chen Z., Wang H., Xu X., Hamdy R., Mc Mullen J.R., Shioi T., Izumo S., Chua B. (2007). Resistin, an adipocytokine, offers protection against acute myocardial infarction. J. Mol. Cell Cardiol., 43: 601-609.
Search in Google Scholar
Gletsu - Miller N., Hansen J.M., Jones D.P., Go Y.M., Torres W.E., Ziegler T.R., Lin E. (2009). Loss of total and visceral adipose tissue mass predicts decreases in oxidative stress after weight-loss surgery. Obesity (Silver Spring), 17: 439-446.
Search in Google Scholar
Hara H., Lin Y.J., Zhu X., Tai H.C., Ezzelarab M., Balamurugan A.N., Bottino R., Houser S.L., Cooper D.K. (2008). Safe induction of diabetes by high-dose streptozotocin in pigs. Pancreas., 36: 31-38.
Search in Google Scholar
Hung H.F., Wang B.W., Chang H., Shyu K.G. (2008). The molecular regulation of resistin expression in cultured vascular smooth muscle cells under hypoxia. J. Hypertens., 26: 2349-2360.
Search in Google Scholar
Iacobellis G., Barbaro G. (2008). The double role of epicardial adipose tissue as pro- and antiinflammatory organ. Horm. Metab. Res., 40: 442-445.
Search in Google Scholar
Iacobellis G., Leonetti F. (2005). Epicardial adipose tissue and insulin resistance in obese subjects. J. Clin. Endocrinol. Metab., 90: 6300-6302.
Search in Google Scholar
Iacobellis G.F., Assael M.C., Ribaudo A., Zappaterreno G., Alessi U., Di Mar-io F., Leonetti F. (2003). Epicardial fat from echocardiography:anew method for visceral adipose tissue prediction. Obes. Res., 11: 304-310.
Search in Google Scholar
Iacobellis G.,di Gioia C.R., Cotesta D., Petramala L., Travaglini C., De San-tis V., Vitale D., Tritapepe L., Letizia C. (2009). Epicardial adipose tissue adiponectin expression is related to intracoronary adiponectin levels. Horm. Metab. Res., 41: 227-231.
Search in Google Scholar
Karlsson S., Ostlund B., Myrs én- Axcrona U., Sundler F., Ahrén B. (2001). Beta cell adaptation to the dexamethasone-induced insulin resistance in rats involves increased glucose responsiveness but not glucose effectiveness. Pancreas., 22: 148-156.
Search in Google Scholar
Karmazyn M., Purdham D.M., Rajapurohitam V., Zeidan A. (2008). Signalling mechanisms underlying the metabolic and other effects of adipokines on the heart. Cardiovasc. Res., 79: 279-286.
Search in Google Scholar
Kawashima J., Tsuruzoe K., Motoshima H., Shirakami A., Sakai K., Hirashi-ma Y., Toyonaga T., Araki E. (2003). Insulin down-regulates resistin m RNAthrough the synthesis of protein(s) that could accelerate the degradation of resistin m RNAin 3T3-L1 adipocytes. Diabetologia, 46: 231-240.
Search in Google Scholar
Kim M., Oh J.K., Sakata S., Liang I., Park W., Hajjar R.J., Lebeche D. (2008). Role of resistin in cardiac contractility and hypertrophy. J. Mol. Cell Cardiol., 45: 270-280.
Search in Google Scholar
Korah T.E., Ibrahim H.H., Badr E.A., El Shafie M.K. (2011). Serum resistin in acute myocardial infarction patients with and without diabetes mellitus. Postgrad. Med. J., 87: 463-467.
Search in Google Scholar
Kremen J.M., Dolinkova J., Krajickova J., Blaha K., Anderlova Z., Lacinova D., Haluzikova L., Bosanska M., Vokurka S., Svacina M., Haluzik M. (2006). Increased subcutaneous and epicardial adipose tissue production of proinflammatory cytokines in cardiac surgery patients: possible role in postoperative insulin resistance. J. Clin. Endocrinol. Metab., 91: 4620-4627.
Search in Google Scholar
Langheim S., Dreas L., Veschini L., Maisano F., Foglieni C., Ferrarello S., Sin-gara G., Zingone B., Alfieri O., Ferrero E., Maseri A., Ruotolo G. (2010). Increased expression and secretion of resistin in epicardial adipose tissue of patients with acute coronary syndrome. Am. J. Physiol. Heart Circ. Physiol., 298: 746-753.
Search in Google Scholar
Lin Y., Rajala M.W., Berger J.P., Moller D.E., Barzilai N., Scherer P.E. (2001). Hyperglycemia- induced production of acute phase reactants in adipose tissue. J. Biol. Chem., 276: 42077-42083.
Search in Google Scholar
Mariappan N., Elks C.M., Sriramula S., Guggilam A., Liu Z., Borkhsenious O., Francis J. (2010). NF-kappa B-induced oxidative stress contributes to mitochondrial and cardiac dysfunction in type IIdiabetes. Cardiovasc. Res., 85: 473-483.
Search in Google Scholar
Mars M.,de Graaf C.,de Groot L.C., Kok F.J. (2005). Decreases in fasting leptin and insulin concentrations after acute energy restriction and subsequent compensation in food intake. Am. J. Clin. Nutr., 81: 570-577.
Search in Google Scholar
Mazurek T., Zhang L., Zalewski A., Mannion J.D., Diehl J.T., Arafat H., Sarov- - Blat L., O ’ Brien S., Keiper E.A., Johnson A.G., Martin J., Goldstein B.J., Shi Y. (2003). Human epicardial adipose tissue isasource of inflammatory mediators. Circulation, 108: 2460-2466.
Search in Google Scholar
Meugnier E., Faraj M., Rome S., Beauregard G., Michaut A., Pelloux V., Chias-son J.L., Laville M., Clement K., Vidal H., Rabasa - Lhoret R. (2007). Acute hyperglycemia inducesaglobal downregulation of gene expression in adipose tissue and skeletal muscle of healthy subjects. Diabetes, 56: 992-999.
Search in Google Scholar
Miles P.D., Romeo O.M., Higo K., Cohen A., Rafaat K., Olefsky J.M. (1997). TNFalpha induced insulin resistance in vivo and its prevention by troglitazone. Diabetes, 46: 1678-1683.
Search in Google Scholar
Mu H., Ohashi R., Yan S., Chai H., Yang H., Lin P., Yao Q., Chen C. (2006). Adipokine resistin promotes in vitro angiogenesis of human endothelial cells. Cardiovasc. Res., 70: 146-157.
Search in Google Scholar
Rangwala S.M., Rich A.S., Rhoades B., Shapiro J.S., Obici S., Rossetti L., La -zar M.A. (2004). Abnormal glucose homeostasis due to chronic hyperresistinemia. Diabetes, 53: 1937-1941.
Search in Google Scholar
Sakoda H., Ogihara T., Anai M., Funaki M., Inukai K., Katagiri H., Fukushi-ma Y., Onishi Y., Ono H., Fujishiro M., Kikuchi M., Oka Y., Asano T. (2000). Dexamethasone- induced insulin resistance in 3T3-L1 adipocytes is due to inhibition of glucose transport rather than insulin signal transduction. Diabetes, 49: 1700-1708.
Search in Google Scholar
Sasayama D., Hori H., Nakamura S., Yamamoto N., Hattori K., Teraishi T., Ota M., Kunugi H. (2014). Increased protein and m RNAexpression of resistin after dexamethasone administration. Horm. Metab. Res., DOI: 10.1055/s-0034-1383651. 10.1055/s-0034-138365125011018
Search in Google Scholar
Sato N., Kobayashi K., Inoguchi T., Sonoda N., Imamura M., Sekiguchi N., Na -kashima N., Schook L.B., Beever J.E., Roger J., Humphray S., Archibald A., Chardon P., Milan D., Rohrer G., Eversole K. (2005). Swine Genome Sequencing Consortium (SGSC):astrategic roadmap for sequencing the pig genome. Comp. Funct. Genom., 6: 251-255.
Search in Google Scholar
Sheng C.H., Di J., Jin Y., Zhang Y.C., Wu M., Sun Y., Zhang G.Z. (2008). Resistin is expressed in human hepatocytes and induces insulin resistance. Endocrine, 33: 135-143.
Search in Google Scholar
Shojima N., Sakoda H., Ogihara T., Fujishiro M., Katagiri H., Anai M., Onis -hi Y., Ono H., Inukai K., Abe M., Fukushima Y., Kikuchi M., Oka Y., Asano T. (2002). Humoral regulation of resistin expression in 3T3-L1 and mouse adipose cells. Diabetes, 51: 1737-1744.
Search in Google Scholar
Stan D., Calin M., Manduteanu I., Pirvulescu M., Gan A.M., Butoi E.D., Simion V., Simionescu M. (2011). High glucose induces enhanced expression of resistin in human U937 monocyte-like cell line by MAPK- and NF-k B-dependent mechanisms; the modulating effect of insulin. Cell Tissue Res., 343: 379-387.
Search in Google Scholar
Stelmanska E., Kmiec Z., Swierczynski J. (2012). The gender- and fat depot-specific regulation of leptin, resistin and adiponectin genes expression by progesterone in rat. J. Steroid. Biochem. Mol. Biol., 132: 160-167.
Search in Google Scholar
Steppan C.M., Bailey S.T., Bhat S., Brown E.J., Banerjee R.R., Wright C.M., Patel H.R., Ahima R.S., Lazar M.A. (2001). The hormone resistin links obesity to diabetes. Nature, 409: 307-312.
Search in Google Scholar
van Raalte D.H., Diamant M. (2014). Steroid diabetes: from mechanism to treatment? Neth. J. Med., 72: 62-72.
Search in Google Scholar
Yki- J ärvinen H. (2004). Insulin therapy in type 2 diabetes: role of the long-acting insulin glargine analogue. Eur. J. Clin. Invest., 34: 410-416.
Search in Google Scholar

Hyperglycemia-induced changes in resistin gene expression in white adipose tissue in piglets

Abstract

Paradigm

My account