Association of the Expression Level of the MYBPC1 Gene in Skeletal Muscle With Marbling Trait in Japanese Black Beef Cattle

Tong, Bin; Muramatsu, Youji; Ohta, Takeshi; Kose, Hiroyuki; Yamashiro, Hideaki; Sugiyama, Toshie; Yamada, Takashisa

doi:10.1515/aoas-2015-0014

Abstract

Marbling characterized by the amount and distribution of intramuscular fat (IMF) in longissimus muscle (LM) and measured as beef marbling score (BMS), is an economically important trait of beef cattle in Japan. The myosin binding protein C, slow type (MYBPC1) gene, involved in efficient energy metabolism and homeostasis during muscle contraction in slow skeletal muscle, has been previously shown to be expressed at different levels in the LM between high-marbled and lowmarbled steer groups using differential-display PCR (ddPCR). In this study, we found that IMF area (%) in the sacrococcygeus muscle (SM) was positively correlated with BMS in the LM in Japanese Black steers (n=22, r=0.941, P<0.0001). This suggested that the IMF area (%) in the SM tends to equate marbling level in the LM. Furthermore, we showed that the MYBPC1 expression level in SM was significantly higher in the Japanese Black steers (n=5) with high BMS than in the Japanese Black steers (n=5) with low BMS (P<0.001). Moreover, correlation analyses showed that the expression level of the MYBPC1 gene was positively correlated with IMF area (%) (n=22, r=0.858, P<0.0001) and BMS (n=22, r=0.769, P<0.0001), indicating the association of MYBPC1 expression level with marbling trait. These results, together with the previous ddPCR result, suggested that high level of MYBPC1 expression may be associated with the development of marbling in Japanese Black beef cattle.

References

Annika M., Carolina E.H., Lars M., Ulf E., Annelie F. (2013). Imaging of neutral lipids by oil red O for analyzing the metabolic status in health and disease. Nat. Protoc., 8: 1149-1154.
Search in Google Scholar
Boylston T.D., Morgan S.A., Johnson K.A., B u s b o om J.R., Wright R.W., Reeves J.J. (1995). Lipid-content and composition of Wagyu and domestic breeds of beef. J. Agric. Food Chem., 43: 1202-1207.
Search in Google Scholar
Brackebusch S.A., McKeith F.K., Carr T.R., McLaren D.G. (1991). Relationship between longissimus composition and the composition of other major muscles of the beef carcass. J. Anim. Sci., 69: 631-640.
Search in Google Scholar
Busboom J.R.,Jeremiah L.E.,Gibson L.L., Johnson K.A., Gaskins C.T., R e e v e s J. J., Wri ght R.W. (1993). Effects of biological source on cooking and palatability attributes of beef produced for the Japanese market. Meat Sci., 35: 241-258.
Search in Google Scholar
Chen Z., Zhao T.J., Li J., Gao Y.S., Meng F.G., Yan Y.B., Zhou H.M. (2011). Slow skeletal muscle myosin-binding protein-C (MYBPC1) mediates recruitment of muscle-type creatine kinase (CK) to myosin. Biochem. J., 436: 437-445.
Search in Google Scholar
Garrett W.N., Hinman N. (1971). Fat content of trimmed beef muscles as influenced by quality grade, yield grade, marbling score and sex. J. Anim. Sci., 33: 948-957.
Search in Google Scholar
Harper G.S., Pethick D.W. (2004). How might marbling begin? Aust. J. Exp. Agric., 44: 653-662.
Search in Google Scholar
Jeong J., Kwon E.G., Im S.K., Seo K.S., Baik M. (2012). Expression of fat deposition and fat removal genes is associated with intramuscular fat content in longissimus dorsi muscle of Korean cattle steers. J. Anim. Sci., 90: 2044-2053.
Search in Google Scholar
JMGA (1988). New Beef Carcass Grading Standards. Japan Meat Grading Association, Tokyo, Japan.
Search in Google Scholar
Jurie C., Cassar-Malek I., Bonnet M., Leroux C., Bauchart D., Boulesteix P., Pethick D.W., Hocquette J.F. (2007). Adipocyte fatty acid-binding protein and mitochondrial enzyme activities in muscles as relevant indicators of marbling in cattle. J. Anim. Sci., 85: 2660-2669.
Search in Google Scholar
Liu Y., Wada R., Yamashita T., Mi Y., Deng C.X., Hobson J.P., Rosenfeldt H.M., Nava V.E., Chae S.S., Lee M.J., Liu C.H., Hla T., Spiegel S., Proia R.L. (2000). Edg-1, the G protein-coupled receptor for sphingosine-1-phosphate, is essential for vascular maturation. J. Clin. Invest., 106: 951-961.
Search in Google Scholar
Matsuishi M., Fujimori M., Okitani A. (2001). Wagyu beef aroma in Wagyu (Japanese Black cattle) beef preferred by the Japanese over imported beef. Anim. Sci. J., 72: 498-504.
Search in Google Scholar
Offer G., Moos C., Starr R. (1973). A new protein of the thick filaments of vertebrate skeletal myofibrils: Extraction, purification and characterization. J. Mol. Biol., 74: 653-676.
Search in Google Scholar
Osawa T., Kuchida K., Hidaka S., Kato T. (2008). Genetic parameters for image analysis traits on M. longissimus thoracis and M. trapezius of carcass cross section in Japanese Black cattle. J. Anim. Sci., 86: 40-46.
Search in Google Scholar
Park B., Whittaker A.D., Miller R.K., Hale D.S. (1994). Predicting intramuscular fat in beef longissimus muscle from speed of sound. J. Anim. Sci., 72: 109-116.
Search in Google Scholar
Pepe F.A., Drucker B. (1975). The myosin filament: III. C-protein. J. Mol. Biol., 99: 609-617.
Search in Google Scholar
Sasaki Y, Nagai K., Nagata Y, Doronbekov K., Nishimura S., Yoshioka S., Fu- jita T., Shiga K., Miyake T., Taniguchi Y., Yamada T. (2006). Exploration of genes showing intramuscular fat deposition-associated expression changes in longissimus muscle. Anim. Genet., 37: 40-46.
Search in Google Scholar
Sato N., Kawakami T., Nakayama A., Suzuki H., Kasahara H., Obinata T. (2003). A novel variant of cardiac myosin-binding protein-C that is unable to assemble into sarcomeres is expressed in the aged mouse atrium. Mol. Biol. Cell., 14: 3180-3191.
Search in Google Scholar
Schmittgen T.D., Livak K.J. (2008). Analyzing real-time PCR data by the comparative C (T) method. Nat. Protoc., 3: 1101-1108.
Search in Google Scholar
Smith S.B., Lunt D.K., Zembayashi M. (2000). Intramuscular fat deposition: The physiological process and the potential for its manipulation. Plains Nutritional Council Spring Conference, Texas A& M Research and Extension Centre, San Antonio Texas, USA, pp. 1-12.
Search in Google Scholar
Tong B., Sasaki S., Muramatsu Y, Ohta T., Kose H., Yamashiro H., Fujita T., Yamada T. (2014). Association of a single nucleotide polymorphism in myosin binding protein C, slow type (MYBPC1) gene with marbling in Japanese Black beef cattle. Anim. Genet., 45: 611-612.
Search in Google Scholar
Wang Y.H.,B o wer N.I., Re verter A.Tan S.H.,De JagerN.WangR., McWilliam S.M., Cafe L.M., Greenwood P.L., Lehnert S.A. (2009). Gene expression patterns during intramuscular fat development in cattle. J. Anim. Sci., 87: 119-130.
Search in Google Scholar
Yamada T., Itoh M., Nishimura S., Taniguchi Y, Miyake T., Sasaki S., Yoshioka S., Fujita T., Shiga K., Morita M., Sasaki Y. (2009). Association of single nucleotide polymorphisms in endothelial differentiation, sphingolipid G-protein-coupled receptor, 1 gene with marbling in Japanese Black beef cattle. Anim. Genet., 40: 209-216.
Search in Google Scholar
Yang X.J., Albrecht E., Ender K., Zhao R.Q., Wegner J. (2006). Computer image analysis of intramuscular adipocytes and marbling in the longissimus muscle of cattle. J. Anim. Sci., 84: 3251-3258.
Search in Google Scholar

Association of the Expression Level of the MYBPC1 Gene in Skeletal Muscle With Marbling Trait in Japanese Black Beef Cattle

Abstract

Paradigm

My account