Molecular Selection Of Tomato And Pepper Breeding Lines Possessing Resistance Alleles Against Tobamoviruses

Hudcovicová, Martina; Korbelová, Erika; Šliková, Svetlana; Klčová, Lenka; Mihálik, Daniel; Kraic, Ján

doi:10.1515/agri-2015-0008

Abstract

Molecular selection among newly created tomato and pepper lines was applied for identification of lines possessing DNA markers linked with the resistant loci against tobamoviruses ToMV, TMV, PaMMV, and PMMoV. Only four tomato lines among 184 had DNA marker linked with the resistant allele Tm-2 conferring homozygosity at this locus. Resistance of these four lines was tested and confirmed also by virological testing by inoculation with TMV strain 0. Simultaneously tested lines heterozygous at this locus expressed full or unbalanced resistance. Fifty-eight out of 62 tested pepper lines had only marker linked to susceptible allele of the locus L. Three lines derived from tobamovirus resistant pepper cultivars Brill and Brilliant expressed marker linked to resistant allele L³, and only one line derived from resistant cultivar Hurricane possessed both markers. Four selected pepper lines declared resistance also after artificial inoculation with the TMV P₀ pathotype. Molecular selection, both in tomato and pepper breeding lines, may be useful in breeding programs directed to tobamovirus resistance.

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Molecular Selection Of Tomato And Pepper Breeding Lines Possessing Resistance Alleles Against Tobamoviruses

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