Molecular Detection of PCV2 And PPV in Pigs in Republic of Srpska, Bosnia and Herzegovina

Lukač, Bojan; Knežević, Aleksandra; Milić, Nenad; Krnjaić, Dejan; Veljović, Ljubiša; Milićević, Vesna; Zorić, Andrea; Đurić, Spomenka; Stanojević, Maja; Nišavić, Jakov

doi:10.1515/acve-2016-0004

Abstract

The presence of porcine circovirus 2 and porcine parvovirus was examined in forty clinical samples of spleen, lymph nodes and lungs originating from non-vaccinated swine by polymerase chain reaction. All animals were reared in extensive livestock farming systems in different geographical districts of Republic of Srpska, Bosnia and Herzegovina. Porcine circovirus 2 DNA was detected in four lymph node and two spleen samples (15%), while porcine parvovirus DNA was identified in five lymph node samples (12.5%). The presence of both viruses was detected in three lymph node samples (7.5%). Partial nucleotide sequence of ORF1 gene of 2 porcine circovirus 2 and VP2 gene of 2 porcine parvovirus isolates was determined. The nucleotide sequences of two PCV2 isolates from RS-BIH included in phylogenetic typing are similar and cluster together with the strain Mantova isolated from domestic pigs in Italy, strains DE006-14 and DE222-13 isolated from pigs in Germany as well as with the strain Jvnan isolated from pigs in China. Also, analyzed PCV2 isolates were partially similar to the strain NIV-C SRB isolated from pigs in Serbia. The nucleotide sequences of two PPV isolates that were included in phylogenetic typing showed a high level of similarity with the strain Challenge isolated from pigs in UK, strain Kresse isolated from pigs in USA and strains 77 and LZ isolated from pigs in China.

References

1. Wei C, Zhang M, Chen Y, Xie J, Huang Z, Zhu W, Xu T, Cao Z, Zhou P, Su S, Zhang G: Genetic evolution and phylogenetic analysis of porcine circovirus type 2 infections in southern China from 2011 to 2012. Infect Genet Evol 2013, 17:87-92.10.1016/j.meegid.2013.03.04123562733
Search in Google Scholar
2. Haley C, Wagner B, Puvanendiran S, Abrahante J, Murtaugh MP: Diagnostic performance measures of ELISA and quantitative PCR tests for porcine circovirus type 2 exposure using Bayesian latent class analysis. Prev Vet Med 2011, 101:79-88.10.1016/j.prevetmed.2011.05.00121632130
Search in Google Scholar
3. Segales J: Porcine circovirus type 2 (PCV2) infections: Clinical signs, pathology and laboratory diagnosis. Virus Res 2012, 164:10-19.10.1016/j.virusres.2011.10.00722056845
Search in Google Scholar
4. Marks FS, Reck J, Almeida LL, Berger M, Corre AMR, Driemeier D, Barcellos D, Guimara JA, Termignoni C, Canal CW: Porcine circovirus 2 (PCV2) induces a procoagulant state in naturally infected swine and in cultured endothelial cells. Vet Microbiol 2010, 141:22-30.10.1016/j.vetmic.2009.08.00419733020
Search in Google Scholar
5. Chang CY, Deng MC, Wang F, Tsai H, Yang C, Chang C, Huang Y: The application of a duplex reverse transcription real-time PCR for the surveillance of porcine reproductive and respiratory syndrome virus and porcine circovirus type 2. J Virol Methods 2014, 201:13-19.10.1016/j.jviromet.2014.01.01624560782
Search in Google Scholar
6. Grau-Rama L, Segales J: Detection of porcine reproductive and respiratory syndrome virus, porcine circovirus type 2, swine infl uenza virus and Aujeszky’s disease virus in cases of porcine proliferative and necrotizing pneumonia (PNP) in Spain. Vet Microbiol 2007, 119:144-151.10.1016/j.vetmic.2006.09.00917070659
Search in Google Scholar
7. Liu JK, Wei CH, Zang XY, Dai AL, Li XH: Multiplex PCR for the simultaneous detection of porcine reproductive and respiratory syndrome virus, classical swine fever virus, and porcine circovirus in pigs. Mol Cell Probes 2013, 27:149-152.10.1016/j.mcp.2013.03.00123524252
Search in Google Scholar
8. Toplak I, Lazić S, Lupulović D, Prodanov-Radulović J, Becskei Z, Došen R, Petrović T: Study of the genetic variability of porcine Circovirus type 2 detected in Serbia and Slovenia. Acta Vet Hungarica 2012, 60:409-420.10.1556/avet.2012.03522903085
Search in Google Scholar
9. Becskei Z, Aleksić - Kovačević S, Rusvai M, Balka G, Jakab C, Petrović T, Knežević M: Distribution of porcine circovirus 2 Cap antigen in the lymphoid tissue of pigs affected by postweaning multisystemic wasting syndrome. Acta Vet Hungarica 2010, 58:483-498.10.1556/avet.58.2010.4.921087918
Search in Google Scholar
10. Cadar D, Dan A, Tombacz K, Lorincz M, Kiss T, Becskei Z, Spinua M, Tuboly T, Csagola A: Phylogeny and evolutionary genetics of porcine parvovirus in wild boars. Infect Genet Evol 2012, 12:1163-1171.10.1016/j.meegid.2012.04.02022575819
Search in Google Scholar
11. Dias AS, Gerber PF, Araujo AS, Auler PA, Gallinari GC, Lobato ZIP: Lack of antibody protection against Porcine circovirus 2 and Porcine parvovirus in naturally infected dams and their offspring. Res Vet Sci 2013, 94:341-345.10.1016/j.rvsc.2012.09.009
Search in Google Scholar
12. Chen HY, Li XK, Cui BA, Wei ZY, Li XS, Wang YB, Zhai L, Wang ZY: A TaqMan-based real-time polymerase chain reaction for the detection of porcine parvovirus. J Virol Methods 2009, 156:84-88.10.1016/j.jviromet.2008.10.029
Search in Google Scholar
13. Ogawa H, Taira O, Hirai T, Takeuchi H, Nagao A, Ishikawa Y, Tuchiya K, Nunoya T, Ueda S: Multiplex PCR and multiplex RT-PCR for inclusive detection of major swine DNA and RNA viruses in pigs with multiple infections. J Virol Methods 2009, 160:210-214.10.1016/j.jviromet.2009.05.010
Search in Google Scholar
14. Nišavić J, Milić N, Knežević A: Laboratorijska dijagnostika virusnih infekcija. In:Izolacija virusa u kulturi ćelija. Beograd, Srbija: Naučna KMD; 2013, 34-36.
Search in Google Scholar
15. Nišavić J, Milić N: Examination of the activity of glycoprotein HN and F antigens of outer envelope of the parainfl uenza virus type 3 by using fusional, hemolytic and hemagglutinating test, in vitro. Acta Vet-Beograd 2006, 56:431-436.10.2298/AVB0606431N
Search in Google Scholar
16. Savić B, Radanović O, Jovičić D, Nešić K, Ivanović S, Stevančević O, Cvetojević Đ, Kasagić D: Survey of infectious agents associated with porcine respiratory disease complex (PRDC) in Serbian swine herds using polymerase chain reaction (PCR) detection. Acta Vet-Beograd 2015, 65:79-88.10.1515/acve-2015-0006
Search in Google Scholar
17. Soares RM, Durigon EL, Bersano JG, Richtzenhain LJ: Detection of porcine parvovirus DNA by the polymerase chain reaction assay using primers to the highly conserved nonstructural protein gene, NS-1. J Virol Methods 1999, 78:191-198.10.1016/S0166-0934(98)00177-3
Search in Google Scholar
18. Henriques AM, Duarte M, Fagulha T, Ramos F, Barros SC, Luis T, Fevereiro M: Molecular study of porcine circovirus type 2 circulating in Portugal. Infect Genet Evol 2011, 11:2162-2172.10.1016/j.meegid.2011.08.02321925289
Search in Google Scholar
19. Ramos N, Mirazo S, Castro G, Arbiza J: Molecular analysis of Porcine Circovirus Type 2 strains from Uruguay: Evidence for natural occurring recombination. Infect Genet Evol 2013, 19:23-31.10.1016/j.meegid.2013.06.01723806516
Search in Google Scholar
20. Chae C: Porcine circovirus 2 and its associated diseases in Korea. Virus Res 2012, 164:107-113.10.1016/j.virusres.2011.10.01322027190
Search in Google Scholar
21. Xu YG, Cui LC, Wang HW, Huo GC, Li SL: Characterization of the capsid protein VP2 gene of a virulent strain NE/09 of porcine parvovirus isolated in China. Res Vet Sci 2013, 94:219-224.10.1016/j.rvsc.2012.09.00323021153
Search in Google Scholar

Molecular Detection of PCV2 And PPV in Pigs in Republic of Srpska, Bosnia and Herzegovina

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