Effect of rutin from tartary buckwheat sprout on serum glucose-lowering in animal model of type 2 diabetes

Lee, Dong-Gi; Jang, Ik Soon; Yang, Kyeong Eun; Yoon, So-Jung; Baek, Sujeong; Lee, Joo Yong; Suzuki, Tatsuro; Chung, Keun-Yook; Woo, Sun-Hee; Choi, Jong-Soon

doi:10.1515/acph-2016-0021

Abstract

This study investigates the anti-diabetic effects of rutin from tartary buckwheat sprout in type 2 diabetes mouse model. The rutin content in tartary buckwheat sprout (TBS) is five times higher than that found in common buckwheat sprout (CBS) as evident from high-performance liquid chromatography analysis. Administration of either rutin or TBS ethanolic extract to diabetes mice decreased the serum glucose level significantly. Rutin down-regulated the expression levels of protein-tyrosine phosphatase 1B; it is negative regulator of insulin pathway, both transcriptionally and translationally in myocyte C2C12 in a dose-dependent manner. In conclusion, rutin can play a critical role in down-regulation of serum glucose level in type 2 diabetes.

References

1. S. T. Ishii, C. Katsumura, K. Shiozuka, K. Ooyauchi, S. Kawasaki, T. Takigawa, Y. Fukushima, M. Tokuji, M. Kinoshita, M. Ohinishi, K. Kawahara and K. Ohba, Anti-inflammatory effect of buckwheat sprouts in lipopolysaccharide-activated human colon cancer cells and mice, Biosci. Biotech. Biochem. 72 (2008) 3148–3157; DOI: 10.1271/bbb.80324.10.1271/bbb.80324
Search in Google Scholar
2. N. Fabjan, I. J. Rode, Z. Kosir, Z. Wang, I. Zhang and I. Kreft, Tartary buckwheat (Fagopyrum tataricum Gaertn.) as a source of dietary rutin and quercetin, J. Agr. Food Chem.51 (2003) 6452–6455; DOI: 10.1021/jf034543e.10.1021/jf034543e
Search in Google Scholar
3. J. M. Kawa, C. G. Taylor and R. Przlybylski, Buckwheat concentrate reduces serum glucose in streptozotocin – diabetic rats, J. Agr. Food Chem. 51 (2003) 7287–7291; DOI: 10.1021/jf0302153.10.1021/jf0302153
Search in Google Scholar
4. C.-C. Lee, W.-H. Hsu, S.-R. Shen, Y.-H. Cheng and S. C. Wu, Fagopyrum tataricum (buckwheat) improved high-glucose–induced insulin resistance in mouse hepatocytes and diabetes in fructoserich diet-induced mice, Exp. Diabetes Res. 2012, 375673; DOI: 10.1155/2012/375673.10.1155/2012/375673
Search in Google Scholar
5. K. H. Egawa, S. Maegawa, K. Shimizu, Y. Morino, M. Nishio, A. T. Bryer-Ash, J. K. Cheung, R. Kolls, A. Kkiawa and A. Kashiwagi, Protein tyrosine phosphatase-1B negatively regulates insulin signaling in L6 myocytes and Fao hepatoma cells, J. Biol. Chem. 276 (2002) 10207–10211; DOI: 10.1074/jbc.M009489200.10.1074/jbc.M009489200
Search in Google Scholar
6. C. L. Liu, Y. S. Chen, J. H. Yang and B. H. Chiang, Antioxidant activity of tartary (Fargopyrum tataricum L. Gaertn.) and common (Fargopyrum esculentum Moench) buckwheat sprouts, J. Agr. Food Chem. 56 (2008) 173–178; DOI: 10.1021/jf072347s.10.1021/jf072347s
Search in Google Scholar
7. J. H. Nam, D.-G. Lee, J. Kwon, C.-W. Choi, S. W. Park, S.-O. Kwon, J. H. Jung, H. C. Park, B. Y. Park, I. S. Jang, W. Y. Bang, C. W. Kim and J.-S. Choi, Comparative proteome analysis of porcine Longissimus dorsi on the basis of pH24 of post-mortem muscle, J. Agr. Sci. 4 (2012) 48–56; DOI: 10.5539/jas.v4n9p48.10.5539/jas.v4n9p48
Search in Google Scholar
8. J. W. Hwang, Y. M. Baek, K. E. Yang, H. S. Yoo, C. K. Cho, Y. W. Lee, J. Park, C. Y. Eom, Z. W. Lee, J. S. Choi and I. S. Jang, Lactobacillus casei extract induces apoptosis in gastric cancer by inhibiting NF-κB and mTOR-mediated signaling, Integr. Cancer Ther. 12 (2012) 165–173; DOI: 10.1177/1534735412442380.10.1177/1534735412442380
Search in Google Scholar
9. L. Bramati, F. Aquilano and P. Pietta, Unfermented rooibos tea: quantitative characterization of flavonoids by HPLC-UV and determination of the total antioxidant activity, J. Agr. Food Chem. 51 (2003) 7472–7474; DOI: 10.1021/jf0347721.10.1021/jf0347721
Search in Google Scholar
10. I. Kreft, N. Fabjan and K. Yasumoto, Rutin content in buckwheat (Fagopyrum esculentum Moench) food materials and products, Food Chem. 98 (2006) 508–512; DOI: 10.1016/j.foodchem.2005.05.081.10.1016/j.foodchem.2005.05.081
Search in Google Scholar
11. I. Musallam, M. Duwayri, R. Shibli and F. Alali, Investigation of rutin content in different plant parts of wild caper (Capparis spinosa L.) populations from Jordan, Res. J. Med. Plant6 (2012) 27–36; DOI: 10.3923/rjmp.2012.27.36.10.3923/rjmp.2012.27.36
Search in Google Scholar
12. E. P. Cai and J. K. Lin, Epigallocatechingallate (EGCG) and rutin suppress the glucotoxicity through activating IRS2 and AMPK signaling in rat pancreatic beta cells, J. Agr. Food Chem. 57 (2009) 9817–9827; DOI: 10.1021/jf902618v.10.1021/jf902618v
Search in Google Scholar

Effect of rutin from tartary buckwheat sprout on serum glucose-lowering in animal model of type 2 diabetes

Abstract

Paradigm

My account