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Expression of autophagic and ubiquitin–proteasome proteins in the peripheral nervous system after nerve injury Cover

Expression of autophagic and ubiquitin–proteasome proteins in the peripheral nervous system after nerve injury

Asian Biomedicine
Volume 13 (2019): Issue 5 (October 2019)
By: Sithiporn Agthong,  Marisa Rodtayoy and  Atitaya Roumwong  
Open Access
|Jun 2020

Figures & Tables

Figure 1

p62 protein in the right (R) (white bar), left proximal (LP) (light gray bar), and left distal (LD) (dark gray bar) sciatic nerves of rats sacrificed at day 1 (D1), day 3 (D3), day 7 (D7), and day 14 (D14) after nerve crush (n = 7 for each time point). Representative immunoblots are shown above. The bar chart demonstrates the average protein level at each time point. Error bars represent SEM. *P < 0.05 vs. R, #P < 0.01 vs. R.
p62 protein in the right (R) (white bar), left proximal (LP) (light gray bar), and left distal (LD) (dark gray bar) sciatic nerves of rats sacrificed at day 1 (D1), day 3 (D3), day 7 (D7), and day 14 (D14) after nerve crush (n = 7 for each time point). Representative immunoblots are shown above. The bar chart demonstrates the average protein level at each time point. Error bars represent SEM. *P < 0.05 vs. R, #P < 0.01 vs. R.

Figure 2

Beclin-1 protein in the right (R) (white bar), left proximal (LP) (light gray bar), and left distal (LD) (dark gray bar) sciatic nerves of rats sacrificed at day 1 (D1), day 3 (D3), day 7 (D7), and day 14 (D14) after nerve crush (n = 7 for each time point). Representative immunoblots are shown above. The bar chart demonstrates the average protein level at each time point. Error bars represent SEM.
Beclin-1 protein in the right (R) (white bar), left proximal (LP) (light gray bar), and left distal (LD) (dark gray bar) sciatic nerves of rats sacrificed at day 1 (D1), day 3 (D3), day 7 (D7), and day 14 (D14) after nerve crush (n = 7 for each time point). Representative immunoblots are shown above. The bar chart demonstrates the average protein level at each time point. Error bars represent SEM.

Figure 3

p62 protein in the right (white bars) and left (light-gray bars) L4-6 dorsal root ganglia of rats sacrificed at day 1 (D1), day 3 (D3), day 7 (D7), and day 14 (D14) after nerve crush (n = 7 for each time point). Representative immunoblots are shown above. The bar chart demonstrates the average protein level at each time point. Error bars represent SEM.
p62 protein in the right (white bars) and left (light-gray bars) L4-6 dorsal root ganglia of rats sacrificed at day 1 (D1), day 3 (D3), day 7 (D7), and day 14 (D14) after nerve crush (n = 7 for each time point). Representative immunoblots are shown above. The bar chart demonstrates the average protein level at each time point. Error bars represent SEM.

Figure 4

Beclin-1 protein in the right (R, white bars) and left (L, gray bars) L4-6 dorsal root ganglia of rats sacrificed at day 1 (D1), day 3 (D3), day 7 (D7), and day 14 (D14) after nerve crush (n = 7 for each time point). Representative immunoblots are shown above. The bar chart demonstrates the average protein level at each time point. Error bars represent SEM.
Beclin-1 protein in the right (R, white bars) and left (L, gray bars) L4-6 dorsal root ganglia of rats sacrificed at day 1 (D1), day 3 (D3), day 7 (D7), and day 14 (D14) after nerve crush (n = 7 for each time point). Representative immunoblots are shown above. The bar chart demonstrates the average protein level at each time point. Error bars represent SEM.

Figure 5

Muscle ring finger-1 protein in the right (R, white bars) and left (L, left bars) L4-6 dorsal root ganglia of rats sacrificed at day 1 (D1), day 3 (D3), day 7 (D7), and day 14 (D14) after nerve crush (n = 7 for each time point). Representative immunoblots are shown above. The bar chart demonstrates the average protein level at each time-point. Error bars represent SEM.
Muscle ring finger-1 protein in the right (R, white bars) and left (L, left bars) L4-6 dorsal root ganglia of rats sacrificed at day 1 (D1), day 3 (D3), day 7 (D7), and day 14 (D14) after nerve crush (n = 7 for each time point). Representative immunoblots are shown above. The bar chart demonstrates the average protein level at each time-point. Error bars represent SEM.

Figure 6

Ubiquitinated protein in the right (R) and left (L) L4-6 dorsal root ganglia of rats sacrificed at day 1 (D1), day 3 (D3), day 7 (D7), and day 14 (D14) after nerve crush (n = 7 for each time point). Representative immunoblots are shown above. The bar chart demonstrates the left/right ratio at each time point. Error bars represent SEM.
Ubiquitinated protein in the right (R) and left (L) L4-6 dorsal root ganglia of rats sacrificed at day 1 (D1), day 3 (D3), day 7 (D7), and day 14 (D14) after nerve crush (n = 7 for each time point). Representative immunoblots are shown above. The bar chart demonstrates the left/right ratio at each time point. Error bars represent SEM.

Figure 7

Representative immunoblot of dorsal root ganglia from rats sacrificed at day 1 (D1), day 3 (D3), day 7 (D7), and day 14 (D14) after nerve crush, probed for beta-actin.
Representative immunoblot of dorsal root ganglia from rats sacrificed at day 1 (D1), day 3 (D3), day 7 (D7), and day 14 (D14) after nerve crush, probed for beta-actin.
DOI: https://doi.org/10.1515/abm-2019-0057 | Journal eISSN: 1875-855X | Journal ISSN: 1905-7415
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Language: English
Page range: 173 - 178
Published on: Jun 4, 2020
Published by: Chulalongkorn University
In partnership with: Paradigm Publishing Services
Publication frequency: 6 issues per year
Keywords:
autophagy,
nerve injury,
proteasome,
ubiquitin
Related subjects:
Medicine,
Assistive professions, nursing,
Basic medical science,
Basic medical science, other,
Clinical medicine,
Clinical medicine, other

© 2020 Sithiporn Agthong, Marisa Rodtayoy, Atitaya Roumwong, published by Chulalongkorn University
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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