Changes in protein patterns of Staphylococcus aureus and Escherichia coli by silver nanoparticles capped with poly (4-styrenesulfonic acid-co-maleic acid) polymer

Tamiyakul, Hathaichanok; Roytrakul, Sittiruk; Jaresitthikunchai, Janthima; Phaonakrop, Narumon; Tanasupawat, Somboon; Warisnoicharoen, Warangkana

doi:10.1515/abm-2019-0039

Figures & Tables

(A) Growth curves of Staphylococcus aureus ATCC 25923 cells and (B) those of Escherichia coli ATCC 25922 cells after exposed (solid line) and unexposed (dashed line) to nanosilver. Error bars indicate standard deviations of the means of n = 4. (C) Patterns of total protein extracted from S. aureus ATCC 25923 and (D) those of E. coli ATCC 25922 after (left) unexposed and (right) exposed to nanosilver at different incubation times (a: 0 min, b: 15 min, c: 30 min, d: 45 min, e: 60 min, f: 120 min, and g: 180 min).

Interaction network of matched categories for (left) Staphylococcus aureus ATCC 25923 and (right) Escherichia coli ATCC 25922. Thicker lines indicate higher confidence and higher number of interactions described by String database.

The identified proteins by LC–MS/MS with known functions of Staphylococcus aureus and Escherichia coli with untreated (control) and nanosilver (NS)-treated conditions

Protein number accession	Protein name	Protein symbol	Function
S. aureus (control)
gi\|170081960	Long-chain fatty acid transport protein	fadL	Molecular transport
S. aureus (NS treated)
gi\|326798469	3-Deoxy-_d-manno-octulosonic-acid transferase	waaA	Metabolic process
S. aureus (control and NS treated)
gi\|1786970	2,3-Bisphosphoglycerate-dependent phosphoglycerate mutase	gpmA	Metabolic process
E. coli (control)
gi\|1786964	Quinolinate synthase A	nadA	Metabolic process
gi\|153844091	Protein YhgF	yhgF	Metabolic process
E. coli (NS treated)
gi\|146319043	Threonine dehydrogenase and related Zn-dependent dehydrogenase	srlB	Metabolic process
gi\|308050024	Flagellar hook-basal body protein	fliE	Metabolic process
gi\|156973219	UDP-N-acetylmuramoyl-_l-alanyl-_d-glutamate synthetase	murD	Metabolic process
gi\|1279404	Peptide transport system ATP-binding protein SapF	sapF	Molecular transport
gi\|323524861	Enoyl-CoA hydratase/isomerase	tdcF	Signal transduction
gi\|1787018	Probable ATP-dependent helicase DinG	dinG	DNA replication
gi\|283778629	DNA topoisomerase type IA Zn finger domain-containing protein	yrdD	Transcription
gi\|1789715	50S ribosomal protein L4	rplD	Translation
E. coli (control and NS treated)
gi\|227357988	Siderophore biosynthesis IucA/IucC family protein	iucA	Metabolic process
gi\|226943115	Isopropylmalate isomerase large subunit	leuC	Metabolic process
gi\|225010785	PhoH family protein	phoH	Metabolic process
gi\|261854682	Phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase	purH	Metabolic process
gi\|319762019	Haloacid dehalogenase (HAD)-superfamily hydrolase	yqaB	Metabolic process
gi\|331007050	Colicin V production protein	cvpA	Molecular transport
gi\|325067458	Oligopeptide ATP-biding cassette (ABC) transporter periplasmic protein	oppA	Molecular transport
gi\|320353100	OmpA/MotB domain-containing protein	motB	Molecular transport
gi\|257459944	Putative TonB-dependent receptor	tonB	Signal transduction
gi\|333984533	TonB-dependent siderophore receptor	yncD	Signal transduction
gi\|312196358	IclR family transcriptional regulator	iclR	Transcription
gi\|384412003	NusA antitermination factor	nusA	Transcription
gi\|85860692	ATP-dependent RNA helicase	rhlB	Transcription
gi\|260893293	Alanyl-tRNA synthetase	alaS	Translation
gi\|227495901	Leucine—tRNA ligase	leuS	Translation
gi\|29653579	50S ribosomal protein L1	rplA	Translation

Changes in protein patterns of Staphylococcus aureus and Escherichia coli by silver nanoparticles capped with poly (4-styrenesulfonic acid-co-maleic acid) polymer

Figures & Tables

Figure 1

Figure 2

The identified proteins by LC–MS/MS with known functions of Staphylococcus aureus and Escherichia coli with untreated (control) and nanosilver (NS)-treated conditions

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