Figure 1
(A) Growth curves of Staphylococcus aureus ATCC 25923 cells and (B) those of Escherichia coli ATCC 25922 cells after exposed (solid line) and unexposed (dashed line) to nanosilver. Error bars indicate standard deviations of the means of n = 4. (C) Patterns of total protein extracted from S. aureus ATCC 25923 and (D) those of E. coli ATCC 25922 after (left) unexposed and (right) exposed to nanosilver at different incubation times (a: 0 min, b: 15 min, c: 30 min, d: 45 min, e: 60 min, f: 120 min, and g: 180 min).
Table 1
The identified proteins by LC–MS/MS with known functions of Staphylococcus aureus and Escherichia coli with untreated (control) and nanosilver (NS)-treated conditions
| Protein number accession | Protein name | Protein symbol | Function | ||
|---|---|---|---|---|---|
| S. aureus (control) | |||||
| gi|170081960 | Long-chain fatty acid transport protein | fadL | Molecular transport | ||
| S. aureus (NS treated) | |||||
| gi|326798469 | 3-Deoxy-d-manno-octulosonic-acid transferase | waaA | Metabolic process | ||
| S. aureus (control and NS treated) | |||||
| gi|1786970 | 2,3-Bisphosphoglycerate-dependent phosphoglycerate mutase | gpmA | Metabolic process | ||
| E. coli (control) | |||||
| gi|1786964 | Quinolinate synthase A | nadA | Metabolic process | ||
| gi|153844091 | Protein YhgF | yhgF | Metabolic process | ||
| E. coli (NS treated) | |||||
| gi|146319043 | Threonine dehydrogenase and related Zn-dependent dehydrogenase | srlB | Metabolic process | ||
| gi|308050024 | Flagellar hook-basal body protein | fliE | Metabolic process | ||
| gi|156973219 | UDP-N-acetylmuramoyl-l-alanyl-d-glutamate synthetase | murD | Metabolic process | ||
| gi|1279404 | Peptide transport system ATP-binding protein SapF | sapF | Molecular transport | ||
| gi|323524861 | Enoyl-CoA hydratase/isomerase | tdcF | Signal transduction | ||
| gi|1787018 | Probable ATP-dependent helicase DinG | dinG | DNA replication | ||
| gi|283778629 | DNA topoisomerase type IA Zn finger domain-containing protein | yrdD | Transcription | ||
| gi|1789715 | 50S ribosomal protein L4 | rplD | Translation | ||
| E. coli (control and NS treated) | |||||
| gi|227357988 | Siderophore biosynthesis IucA/IucC family protein | iucA | Metabolic process | ||
| gi|226943115 | Isopropylmalate isomerase large subunit | leuC | Metabolic process | ||
| gi|225010785 | PhoH family protein | phoH | Metabolic process | ||
| gi|261854682 | Phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase | purH | Metabolic process | ||
| gi|319762019 | Haloacid dehalogenase (HAD)-superfamily hydrolase | yqaB | Metabolic process | ||
| gi|331007050 | Colicin V production protein | cvpA | Molecular transport | ||
| gi|325067458 | Oligopeptide ATP-biding cassette (ABC) transporter periplasmic protein | oppA | Molecular transport | ||
| gi|320353100 | OmpA/MotB domain-containing protein | motB | Molecular transport | ||
| gi|257459944 | Putative TonB-dependent receptor | tonB | Signal transduction | ||
| gi|333984533 | TonB-dependent siderophore receptor | yncD | Signal transduction | ||
| gi|312196358 | IclR family transcriptional regulator | iclR | Transcription | ||
| gi|384412003 | NusA antitermination factor | nusA | Transcription | ||
| gi|85860692 | ATP-dependent RNA helicase | rhlB | Transcription | ||
| gi|260893293 | Alanyl-tRNA synthetase | alaS | Translation | ||
| gi|227495901 | Leucine—tRNA ligase | leuS | Translation | ||
| gi|29653579 | 50S ribosomal protein L1 | rplA | Translation | ||
Figure 2
Interaction network of matched categories for (left) Staphylococcus aureus ATCC 25923 and (right) Escherichia coli ATCC 25922. Thicker lines indicate higher confidence and higher number of interactions described by String database.