Evidence of growth hormone effect on plasma leptin in diet-induced obesity and diet-resistant rats Cover

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Evidence of growth hormone effect on plasma leptin in diet-induced obesity and diet-resistant rats

Asian Biomedicine

Volume 12 (2018): Issue 5 (October 2018)

By: Sutharinee Likitnukul, Sarinee Kalandakanond-Thongsong and Sumpun Thammacharoen

Open Access

|Oct 2019

Figures & Tables

The experimental procedure is demonstrated. After the obesity induction period, IPGTT was performed before GH or saline treatment (at day -7). Then, the rats were injected with either GH or saline for 3 days and blood samples were collected for plasma hormones and FFA measurement. The white and black bars indicate light and dark phases, respectively (12h/12h light/dark cycle).FFA, free fatty acid; GH, growth hormone; IPGTT, intraperitoneal glucose tolerance test

The effect of short-term GH treatment on body adiposity and leptin per fat mass. (A) There was no effect of GH on body adiposity in all rats, and HC-O rats had the highest fat mass compared to HC-R and control rats (***P < 0.001, **P < 0.01). (B) There was a significant effect of GH on leptin secretion per fat mass in HC-O rats.*The leptin per fat mass of GH-treated HC-O rats was significantly lower than that from saline treatment, P < 0.05GH, growth hormone; HC-O, hypercaloric diet obese; HC-R, hypercaloric diet resistance

The effect of short-term GH treatment on meal-induced plasma leptin. (A) There was no effect of GH on meal-induced plasma leptin, which was demonstrated as leptin difference of pre- and post-meal plasma leptin. (B) The energy intake from meal-induced plasma leptin experiment revealed that HC-O rats had the highest energy intake compared to control and HC-R rats.*The energy intake of HC-O rats was significantly different from others, P < 0.05GH, growth hormone; HC-O, hypercaloric diet obese; HC-R, hypercaloric diet resistance

The effect of short-term GH treatment on the fasting effect on plasma leptin (leptin difference). There was a significant difference of the effect of GH treatment in the control and HC-R groups.*GH effect is significantly different from saline treatment, P < 0.05.GH, growth hormone; HC-R, hypercaloric diet resistance

The effect of short-term GH treatment on the surrogate indexes for insulin receptor sensitivity. (A) The calculated HOMA-IR revealed the significant GH effect in HC-R rats. (B) For the Adipo-IR index, GH treatment could increase this index in HC-R rats.*GH effect is significantly different from saline treatment, P < 0.05.Adipo-IR, adipose tissue insulin resistance; GH, growth hormone; HC-R, hypercaloric diet resistance; HOMA-IR, homeostasis model of insulin resistance.

The effect of short-term GH treatment on basal plasma leptin at 24 and 32 h after GH injection

Time after the first injection	Basal plasma leptin (ng/mL)
	Control	HC-R	HC-O
Saline
24 h	16.98 ± 0.7	18.93 ± 2.7	21.16 ± 1.0
32 h	17.94 ± 1.2	18.47 ± 1.9	29.20 ± 1.8*
GH
24 h	16.89 ± 0.8	15.74 ± 0.9	17.00 ± 1.8
32 h	17.44 ± 2.5	18.38 ± 0.8	21.55 ± 2.0*^,#

The effect of short-term GH treatment on fasting plasma insulin, glucose, and FFA

	Control	HC-R	HC-O
Insulin (ng/mL)
Saline	2.96 ± 0.5	2.39 ± 0.7	5.12 ± 0.8
GH	6.09 ± 1.0^†	6.26 ± 1.4^†	6.92 ± 1.2^†
Glucose (mg/dL)
Saline	131.50 ± 6.8	126.00 ± 6.0	131.50 ± 4.0
GH	135.20 ± 3.0	126.80 ± 12.8	123.30 ± 7.7
FFA (mmol/L)
Saline	0.55 ± 0.13	0.29 ± 0.06	0.60 ± 0.14
GH	0.45 ± 0.10	0.81 ± 0.12	0.67 ± 0.17

Characteristics of the control, HC-R, and HC-O rats in the current experiments

	Control (n = 12)	HC-R (n = 9)	HC-O (n = 12)
Initial BW (g)	441.02 ± 2.93^a	420.19 ± 7.58^b	447.33 ± 5.40^a
Final BW (g)	503.68 ± 3.41^b	508.27 ± 7.73^b	574.52 ± 7.98^a
BW gain (g)	62.67 ± 1.93^c	88.08 ± 4.68^b	127.19 ± 5.80^a
BW gain/day	1.57 ± 0.05^c	2.20 ± 0.12^b	3.18 ± 0.14^a
(g/day)
Body fat mass (%)	11.25 ± 0.30^c	12.92 ± 0.41^b	14.97 ± 0.31^a
AUC-IPGTT	24667 ± 1303^b	24304 ± 1198^b	28411 ± 1242^a

DOI: https://doi.org/10.1515/abm-2019-0023 | Journal eISSN: 1875-855X | Journal ISSN: 1905-7415

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Language: English

Page range: 219 - 228

Published on: Oct 1, 2019

Published by: Chulalongkorn University

In partnership with: Paradigm Publishing Services

Publication frequency: 6 issues per year

Keywords:

adipose tissue,

growth hormone,

insulin resistance,

Related subjects:

Assistive professions, nursing,

Basic medical science,

Basic medical science, other,

Clinical medicine,

Clinical medicine, other

© 2019 Sutharinee Likitnukul, Sarinee Kalandakanond-Thongsong, Sumpun Thammacharoen, published by Chulalongkorn University
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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