Table 1
Demographic data of patients with squamous cell carcinoma of the head and neck (HNSCC)
| Sex | Age (y) | Organ | Histological grade | Clinical stage |
|---|---|---|---|---|
| Microdissected HNSCC | ||||
| M | 51 | Floor of mouth | 2 | 2 |
| F | 62 | Tongue | 1 | 1 |
| M | 65 | Floor of mouth | 2 | 3 |
| F | 62 | Tongue | 3 | 1 |
| M | 67 | Gingiva | 1 | 2 |
| M | 50 | Gingiva | 1 | 2 |
| M | 61 | Tongue | 2 | 4 |
| M | 37 | Tongue | 2 | 2 |
| M | 56 | Tongue | 3 | 4 |
| N0 (no metastasis) | ||||
| 11 | M | Tongue | 2 | 4 |
| 67 | F | Buccal mucosa | 1 | 3 |
| 47 | F | Tongue | 1 | 2 |
| 53 | F | Tongue | 1 | 1 |
| 51 | M | Floor of mouth | 2 | 3 |
| 83 | F | Lip | 2 | 4 |
| 70 | M | Soft palate | 1 | 3 |
| 48 | F | Soft palate | 1 | 4 |
| 72 | M | Palate | 2 | 2 |
| 38 | F | Tongue | 2 | 2 |
| 46 | F | Tongue | 1 | 1 |
| 65 | M | Buccal mucosa | 2 | 3 |
| 81 | M | Tongue | 2 | 1 |
| 72 | F | Buccal mucosa | 2 | 2 |
| 63 | M | Tongue | 2 | 1 |
| Nx (metastasis) | ||||
| 52 | M | Tongue | 1 | 1 |
| 57 | M | Soft palate | 3 | 3 |
| 54 | M | Soft palate | 2 | 4 |
| 56 | M | Tongue | 2 | 4 |
| 79 | F | Palate | 3 | 4 |
| 74 | M | Buccal mucosa | 1 | 4 |
| 42 | M | Tongue | 3 | 4 |
| 66 | M | Soft palate | 2 | 4 |
| 51 | M | Tongue | 2 | 4 |
| 66 | F | Buccal mucosa | 1 | 2 |
| 58 | M | Tongue | 2 | 4 |
| 67 | M | Tongue | 2 | 4 |
| 60 | M | Buccal mucosa | 1 | 4 |
| 69 | M | Gingiva | 2 | 4 |
| 34 | M | Tongue | 1 | 4 |
| 64 | F | Tongue | 2 | 3 |
| 61 | M | Soft palate | 2 | 3 |
| 84 | F | Lower lip | 2 | 4 |
[i] Histological grade: 1 = well-differentiated, 2 = moderately-differentiated, 3 = poorly-differentiated
Figure 1
SHP1-P2 methylation. (A) Percentage of SHP1-P2 methylation comparisons in the 5 sample groups. (B) Comparison of LN– Nx and LN+ Nx. A significant difference was observed between LN– Nx and LN+ Nx (P < 0.0001). (C) ROC curve analysis to distinguish LN N0 from LN– Nx. The curve showed AUC = 0.637 with a sensitivity of 66.67% and a specificity of 60%. AUC, area under the curve; HNSCC, squamous cell carcinoma of the head and neck; LN N0, nonmetastatic HNSCC lymph nodes; LN–Nx = lymph nodes that are histologically negative for tumor cells in patients with metastatic HNSCC; LN+ Nx, lymph nodes that are histologically positive for tumor cells in metastatic HNSCC; ROC, receiver operating characteristic; SCC, squamous cell carcinoma; SHP1-P2, Src homology region 2 domain-containing protein-tyrosine phosphatase 1 promoter 2 patients with
Table 2
Percentage of SHP1-P2 methylation
| Group | n | SHP1-P2 methylation (%, mean ± SD) |
|---|---|---|
| Tonsil | 10 | 10.27 ± 4.05 |
| LN N0 | 15 | 9.99 ± 6.61 |
| LN– Nx | 18 | 14.49 ± 10.03 |
| LN+ Nx | 18 | 41.01 ± 24.51 |
| SCC | 9 | 61.31 ± 17.00 |
[i] HNSCC, squamous cell carcinoma of the head and neck; LN N0, nonmetastatic HNSCC lymph nodes, LN– Nx, lymph nodes that are histologically negative for tumor cells in patients with metastatic HNSCC; LN– Nx, lymph nodes that are histologically positive for tumor cells in patients with metastatic HNSCC; SCC, squamous cell carcinoma; SHP1-P2, Src homology region 2 domain-containing protein-tyrosine phosphatase 1 promoter 2