Table 1
Wheel-running activity recordings. Both alpha and phase shifts indicated are calculated relative to endogenous free-running period.
| Group | P | ||
|---|---|---|---|
| Wild-type | Mutants | ||
| Alpha in LD (h) | 11.35 ± 0.62 | 12.12 ± 0.21 | ns |
| Phase angle in LD (min) | -49 ± 23.15 | 9.4 ± 2.92 | 0.04 |
| Free-running period (h) | |||
| Before light pulse | 23.57 ± 0.11 | 23.93 ± 0.03† | 0.01 |
| After light pulse | 23.57 ± 0.10 | 24.02 ± 0.03† | 0.002 |
| Alpha in DD (circadian h) | 12.35 ± 0.38 | 14.88 ± 0.88 | 0.04 |
| Amplitude in DD (%) | 97.3 ± 0.9 | 97.4 ± 0.5 | ns |
| Amplitude/Alpha (%/h) | 7.90 ± 0.27 | 6.62 ± 0.35 | 0.03 |
| Phase delays in response to LP at CT15 (circadian h) | 1.90 ± 0.34 | 2.92 ± 0.26 | 0.04 |
| Reentrainment to 6 h advance of LD cycle (days) | 8.7 ± 0.3 | 8.2 ± 0.4 | ns |
| Reentrainment to 6 h delay (days) | 2.0 ± 0.6 | 2.4 ± 0.4 | ns |
Phase angle in LD refers to the difference between the time of lights off and activity onset (negative values indicate animals start running before lights off). All data are expressed as Mean ± SEM. P values correspond to Student's t test. † Difference between these two results is not significant according to paired t test analysis.
Figure 1
Representative actograms of a) WT and b) Lgals1-/- type mice. Stars indicate day and time of light pulses at CT15. Clear differences in free-running period and alpha can be appreciated (see Table 1). Moreover, after light pulse at CT 15 (i.e., 3 hours after activity onset under DD), a larger phase delay can be seen in Lgals1-/- mice (see Table 1 for details).
Figure 2
a) Average amount of relative activity (see Methods for details) during each third of alpha, as determined from individual relative activity waveforms under free running conditions. b) Average waveforms for each group, adjusted to tau period. Time point 0 represents CT12. All error bars represent mean ± SEM.