Figure 1
Raster actograms of locomotor activity of representative mice of the B6 and AhR strains. Locomotor activity was monitored by infrared motion detectors. Each line of recorded activity is 48 hr. Each pair of days is plotted beneath the previous pair of days. Activity is indicated by the height of the narrow histograms each 10 min wide.
Figure 2
Circadian period in hours of B6 and AhR strains of inbred mice. Error bars represent the SEM.
Figure 3
Daily profile of locomotor activity of representative mice of the B6 and AhR strains in LD 12:12 as a function of circadian time. Profiles were generated in the "Activity Profile" module in Clocklab (Actimetrics Corp, Evanston, IL) which averaged the activity profiles for the last eight days under LD 12:12. The gray arrow shows the placement of the siesta. The dark line indicates the mean, the shaded areas are SEM.
Figure 4
Circadian period in hours of B6, C12A, and A/J strains of inbred mice. Error bars represent the SEM.
Table 1
Phenotype and genotype of AhR and B6 mice
| AhR | B6 | ||
| Circadian period (h) | 23.83 ± 0.02 | 23.96 ± 0.02 | |
| Marker | Mbp | ||
| D12Mit242 | 30.8 | B6:B6 | B6:B6 |
| D12Mit60 | 35.4 | D2:D2 | B6:B6 |
| D12Mit153 | 35.8 | D2:D2 | B6:B6 |
| rs29213248 | 39.3 | D2:D2 | B6:B6 |
| rs29155751 | 40.0 | D2:D2 | B6:B6 |
| rs29161407 | 40.9 | D2:D2 | B6:B6 |
| D12Mit2 | 42.5 | B6:B6 | B6:B6 |
B6:B6 – homozygous for C57BL/6J alleles
D2:D2 – homozygous for DBA/2J alleles
Mbp – position from NCBI Build 36.1
Table 2
Candidate QTG in the Cplaq10 genomic region
| Gene | Start (Mbp) | Diff expr | Cis-reg | ncSNP | Circ cyc | Ensembl description |
| Ahr | 36.08 | No | 6 SNP | No | aryl-hydrocarbon receptor | |
| Meox2 | 37.62 | No | 1 SNP | No | mesenchyme homeobox 2 | |
| Arl41 | 40.54 | No | No | Yes | ADP-ribosylation factor-like 4A | |
| A530016O06Rik | 37.75 | No | No | Yes | RIKEN cDNA A530016O06 gene | |
| Ifrd1 | 40.71 | No | No | Yes | interferon-related developmental regulator 1 | |
| Zpf277 | 40.83 | Yes | Yes | 2 SNP | Yes | zinc finger protein 277 |
Start (Mbp): from NCBI Build 36.1
Diff expr: Differential expression in whole brain B6 vs D2
Cis-reg: Cis-regulated
ncSNP: non-synonymous coding SNP
Circ cyc: Circadian cycling in liver