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Investigation of a non-invasive method of assessing the equine circadian clock using hair follicle cells Cover

Investigation of a non-invasive method of assessing the equine circadian clock using hair follicle cells

Open Access
|Oct 2012

Figures & Tables

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Figure 1

24-h profiles of mRNA expression. Plotted are mRNA levels of the candidate genes relative to reference genes ACTB and HPRT1 in equine hair follicles. Data are presented as means ± SEM. Black and white bars indicate hours of darkness and light, respectively. ANOVA revealed significant 24-h variation for PER1, PER2 and DBP (p = .024, p = .02 and p = .03;, respectively) whereas Cosinor analysis confirmed a significant 24-h component for PER1, DBP and NR1D2 (p = .002, p = .0331 and p=.03;, respectively.

Table 1

Results of cosinor analyses of mRNA expression profiles

Gene transcript Robustness (%) Acrophase (24 h) Zeitgeber Time (ZT) p value
ARNTL 0n/an/a 0.3066

For each gene transcript the robustness values, acrophase time, corresponding Zeitgeber Time and and significance values (p) are presented.

Language: English
Published on: Oct 5, 2012
Published by: Ubiquity Press
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year

© 2012 Lisa M Watts, John A Browne, Barbara A Murphy, published by Ubiquity Press
This work is licensed under the Creative Commons Attribution 4.0 License.