Morphological and Molecular Characterization of Phoma complanata, a New Causal Agent of Archangelica officinalis Hoffm. in Poland

ZIMOWSKA, BEATA; ZALEWSKA, EWA DOROTA; DOROTA KRÓL, EWA; FURMAŃCZYK, AGNIESZKA

doi:10.5604/01.3001.0010.7886

Abstract

The paper concerns the fungus Phoma complanata, isolated for the first time in Poland, from the roots and umbels of angelica (Archangelica officinalis) in 2009. The morphology of fungal isolates was tested on standard culture media. Moreover, the sequence analysis of ITS regions was conducted. Morphological similarity of P. complanata Polish isolates to the reference isolate obtained from CBS culture collection was determined and together with the molecular analysis confirmed the affiliation of the fungus to the species.

References

Aveskamp M.M., J. De Gruyter and P.W. Crous. 2008. Biology and recent developments in the systematics of Phoma, a complex genus of major quarantine significance. Fungal Diversity 31: 1–18.
Search in Google Scholar Back to article
Aveskamp M.M, J. De Gruyter, J.H.C Woudenberg, G.J.M. Verkley and P.W. Crous. 2010. Highlights of Didymellaceae: A polyphasic approach to characterise Phoma and related pleosporalean genera. Studies in Mycology 65: 1–60.10.3114/sim.2010.65.01283621020502538
Search in Google Scholar Back to article
Balmas V., B. Scherm, S. Ghignone, A.O.M. Salem, S.O. Cacciol and Q. Migheli 2005. Characterization of Phoma tracheiphila by RAPD-PCR, microsatellite-primed PCR Eur. J. Plant Pathol. and ITS rDNA sequencing and development of specific primers for in planta PCR detection. Eur. J. Plant Pathol. 111: 235–247.10.1007/s10658-004-4173-x
Search in Google Scholar Back to article
Boerema G.H. and G.J. Bollen. 1975. Conidiogenesis and conidial septation as differentiating criteria between Phoma and Ascochyta. Persoonia 8: 111–144.
Search in Google Scholar Back to article
Boerema G.H., J. De Gruyter, M.E. Noordeloos and M.E.C. Hamers. 2004. Phoma identification manual differentiation on specific and intra-specific taxa in culture, p. 470. CAB Publishing.10.1079/9780851997438.0000
Search in Google Scholar Back to article
Błaszczyk L., P. Popiel, J. Chełkowski, G. Koczyk, G.J. Samuels, K. Sobieralski and M. Siwulski. 2011. Species diversity of Trichoderma in Poland. J. App. Genetics 52: 233–243.10.1007/s13353-011-0039-z308880321465156
Search in Google Scholar Back to article
Chen Q., J.R. Jiang, G.Z. Zhang, L. Cai, and P.W. Crous. 2015. Resolving the Phoma enigma. Studies in Mycology 82: 137–217.10.1016/j.simyco.2015.10.003477427326955202
Search in Google Scholar Back to article
De Gruyter J. 2012. PhD Thesis. Revised taxonomy of Phoma and allied genera, p. 181. Wageningen University, Wageningen, NL.
Search in Google Scholar Back to article
Farr D.F., G.F. Bills, G.P. Chamuris and A.Y. Rossman. 1995. Fungi on plants products in the United States. American Phytopath. Society.
Search in Google Scholar Back to article
Frisvad J.C., B. Anderson and U. Thrane. 2008. The use of secondary metabolite profiling in chemotaxonomy of filamentous. Mycol. Res. 112: 231–240.10.1016/j.mycres.2007.08.01818319145
Search in Google Scholar Back to article
Kirk P.M., P.F. Cannon, D.W. Minter and J.A. Stalpers. 2008. Ainsworth and Bisby’s dictionary of the Fungi, p. 508. 10^th edition. CABI Europe-UK, CAB International, Wallingford.10.1079/9780851998268.0000
Search in Google Scholar Back to article
Koike S.T., K.V. Subbarao, G.J.M. Verkley, D. Fogle and T.M. O’Neill. 2006. Phoma basal rot of romaine lettuce in California caused by Phoma exigua: Occurrence, characterization and control. Plant Disease 90: 1268–1275.
Search in Google Scholar Back to article
Rai M.K., V.V. Tivari, L. Irinyi and G.J. Köviecs. 2014. Advances in taxonomy of genus Phoma: polyphyletic nature and role of phenotypic traits and molecular systematics. Indian J. Microbiol. 54(2): 123–128.10.1007/s12088-013-0442-8418848625320410
Search in Google Scholar Back to article
Tiwari V.V., A.K. Gade and M. Rai. 2013. A study of phylogenetic variations among Indian Phoma tropica species by RAPD-PCR and ITS-rDNA sequencing. Ind. J. Biotechnol. 12: 187–194.
Search in Google Scholar Back to article
Uddin W., K.L. Stevenson, R.A. Pardo-Schultheiss and S.A. Rehner. 1998. Pathogenic and molecular characterization of three Phomopsis isolates from peach, plum and Asian pear. Plant Disease 82(7): 732–737.10.1094/PDIS.1998.82.7.73230856940
Search in Google Scholar Back to article
White T.J., T. Bruns, S. Lee and J. Taylor. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics, pp. 315–322. In: PCR protocols; a quide to methods and amplifications Innis M.A., D.H. Geefand, S.S. Sninsky and T.J. White (eds). Academic Press, San Diego.10.1016/B978-0-12-372180-8.50042-1
Search in Google Scholar Back to article
Woudemberg J.H.C., M.M. Aveskamp, J. de Gruyter, A.G. Spiers and P.W. Crous. 2009. Multiple Didymella teleomorphs are linked to the Phoma clematidina morphotype. Persoonia 22: 56-62.10.3767/003158509X427808278954120198138
Search in Google Scholar Back to article
Zalewska E., Z. Machowicz-Stefaniak and E. Król. 2013. Occurrence of fungi on the plants of angelica (Archangelica officinalis Hoffm.). Acta Sci. Pol. Hortorum Cultus 12(2): 107–121.
Search in Google Scholar Back to article

Morphological and Molecular Characterization of Phoma complanata, a New Causal Agent of Archangelica officinalis Hoffm. in Poland

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