Polymorphism of COMT Val158Met is associated with inhalant use and dependence: a Thai substance dependence treatment cohort

Intharachuti, Wichaporn; Ittiwut, Rungnapa; Listman, Jenifer; Verachai, Viroj; Mutirangura, Apiwat; Malison, Robert T.; Kalayasiri, Rasmon

doi:10.5372/1905-7415.0604.089

Abstract

Background: Inhalants are abused by adolescents worldwide, but genetic markers of inhalant use or dependence are poorly understood.

Objective: We assessed the frequency and association of a functional polymorphism in the gene encoding catechol-O-methyltransferase (COMT Val158Met) in inhalant-dependent (ID) subjects and inhalant users (IU).

Methods: Demographic and diagnostic data were collected by interviewing 456 Thai-speaking methamphetamine (MA) users by using the Semi-Structured Assessment for Drug Dependence and Alcoholism (SSADDA). COMT Val158Met (rs4680) genotyping was acquired by restriction fragment length polymorphism. Individuals with ID or IU were compared with non-ID or non-IU by using a χ² test. After that, factors associated with ID or IU were analyzed by logistic regression. Blood samples from 217 healthy blood donors were used as controls for ID and IU in the allele frequency comparison. Deviation from Hardy-Weinberg Equilibrium Expectations (HWEE) was also tested.

Results: Out of 456 MA users, deviation from HWEE was observed in IU but not in ID, non-ID or from the total sample. The “Met” allele was significantly associated with ID (p = 0.02) and IU (p = 0.002) among MA users, but not in allele frequency comparisons when compared to the healthy control group (p > 0.1). With respect to logistic regression analysis, homo or heterozygosity for the “Met” allele, male sex, younger age, lower level of education, a major depressive episode (MDE), and alcohol dependence were associated with ID. Analyses of IU vs. non-IU yielded the same results except for age and MDE. In addition, individuals with MA-induced paranoia (MIP) were more likely to have used inhalants at least once in their lifetime compared to those without.

Conclusion: COMT 158Met, male sex, younger age, lower level of education, MDE, MIP, and alcohol dependence increased risk for inhalant use and/or dependence.

References

1. Wu LT, Ringwalt CL. Inhalant use and disorders among adults in the United States. Drug Alcohol Depend. 2006; 85:1-11.10.1016/j.drugalcdep.2006.01.017
Open DOI Search in Google Scholar Back to article
2. Yucel M, Takagi M, Walterfang M, Lubman DI. Toluene misuse and long-term harms: a systematic review of the neuropsychological and neuroimaging literature. Neurosci Biobehav Rev. 2008; 32:910-26.10.1016/j.neubiorev.2008.01.006
Open DOI Search in Google Scholar Back to article
3. Hernandez-Avila CA, Ortega-Soto HA, Jasso A, Hasfura-Buenaga CA, Kranzler HR. Treatment of inhalant-induced psychotic disorder with carbamazepine versus haloperidol. Psychiatric services. 1998; 49:812-6.10.1176/ps.49.6.812
Open DOI Search in Google Scholar Back to article
4. Okudaira K, Yabana T, Takahashi H, Iizuka H, Nakajima K, Saito A. Inhalant abusers and psychiatric symptoms. Seishin Shinkeigaku Zasshi. 1996; 98: 203-12.
Search in Google Scholar Back to article
5. Wada K, Nakayama K, Koishikawa H, Katayama M, Hirai S, Yabana T, et al. Symptomatological structure of volatile solvent-induced psychosis: is “solvent psychosis” a discernible syndrome. Nihon Arukoru Yakubutsu Igakkai Zasshi. 2005; 40:471-84.
Search in Google Scholar Back to article
6. Anderson CE, Loomis GA. Recognition and prevention of inhalant abuse. American family physician. 2003; 68: 869-74.
Search in Google Scholar Back to article
7. Bale AS, Tu Y, Carpenter-Hyland EP, Chandler LJ, Woodward JJ. Alterations in glutamatergic and GABAergic ion channel activity in hippocampal neurons following exposure to the abused inhalant toluene. Neuroscience. 2005; 130:197-206.10.1016/j.neuroscience.2004.08.040
Open DOI Search in Google Scholar Back to article
8. Medina-Mora ME, Real T. Epidemiology of inhalant use. Curr Opin Psychiatry. 2008; 21:247-51.10.1097/YCO.0b013e3282fc9875
Open DOI Search in Google Scholar Back to article
9. Konghom S, Verachai V, Srisurapanont M, Suwanmajo S, Ranuwattananon A, Kimsongneun N, et al. Treatment for inhalant dependence and abuse (Review). The Cochrane Library. 2010.10.1002/14651858.CD007537.pub2
Search in Google Scholar Back to article
10. Ridenour TA. Inhalants: not to be taken lightly anymore. Curr Opin Psychiatry. 2005; 18:243-7.10.1097/01.yco.0000165593.52811.cd
Open DOI Search in Google Scholar Back to article
11. Winqvist R, Lundstrom K, Salminen M, Laatikainen M, Ulmanen I. The human catechol-O-methyltransferase (COMT) gene maps to band q11.2 of chromosome 22 and shows a frequent RFLP with BglI. Cytogenet Cell Genet. 1992; 59:253-7.10.1159/000133262
Open DOI Search in Google Scholar Back to article
12. Lachman HM, Morrow B, Shprintzen R, Veit S, Parsia SS, Faedda G, et al. Association of codon 108/158 catechol-O-methyltransferase gene polymorphism with the psychiatric manifestations of velo-cardiofacial syndrome. Am J Med Genet. 1996; 67:468-72.10.1002/(SICI)1096-8628(19960920)67:5<;468::AID-AJMG5>3.0.CO;2-G
Search in Google Scholar Back to article
13. Shield AJ, Thomae BA, Eckloff BW, Wieben ED, Weinshilboum RM. Human catechol O-methyltransferase genetic variation: gene resequencing and functional characterization of variant allozymes. Mol Psychiatry. 2004; 9:151-60.10.1038/sj.mp.4001386
Open DOI Search in Google Scholar Back to article
14. Axelrod J, Tomchick R. Enzymatic O-methylation of epinephrine and other catechols. J Biol Chem. 1958; 233:702-5.10.1016/S0021-9258(18)64731-3
Search in Google Scholar Back to article
15. Ucok A, Ozturk M, Duman Z, Saruhan-Direskeneli G. COMT Val158Met polymorphism is related with interpersonal problem solving in schizophrenia. European Psychiatry. 2010; 25: 320-2.10.1016/j.eurpsy.2010.01.01220570494
Open DOI Search in Google Scholar Back to article
16. Benedetti F, Dallaspezia S, Colombo C, Lorenzi C, Pirovano A, Smeraldi E. Association between catechol-O-methyltransferase Val(108/158)Met polymorphism and psychotic features of bipolar disorder. Journal of Affective Disorders. 2010; 125: 341-4.10.1016/j.jad.2010.01.00520122740
Search in Google Scholar Back to article
17. Lohoff FW, Weller AE, Bloch PJ, Nall AH, Ferraro TN, Kampman KM, et al. Association between the catechol-O-methyltransferase Val158Met polymorphism and cocaine dependence. Neuropsychopharmacology. 2008; 33:3078-84.10.1038/npp.2008.126258321418704099
Open DOI Search in Google Scholar Back to article
18. Administrative Committee on Substance Abuse Academic Network (ACSAN): National Household Survey on Substance Abuse 2007. Edited by Bangkok Office of the Narcotics Control Board; 2007.
Search in Google Scholar Back to article
19. Malison RT, Kalayasiri R, Sanichwankul K, Sughondhabirom A, Mutirangura A, Pittman B, et al. Inter-rater reliability and concurrent validity of DSM-IV opioid dependence in a Hmong isolate using the Thai version of the Semi-Structured Assessment for Drug Dependence and Alcoholism (SSADDA). Addictive Behaviors. 2011; 36:156-60.10.1016/j.addbeh.2010.08.031298166220888699
Open DOI Search in Google Scholar Back to article
20. Kalayasiri R, Mutirangura A, Verachai V, Gelernter J, Malison RT. Risk factors for methamphetamineinduced paranoia and latency of symptom onset in a Thai drug treatment cohort. Asian Biomed. 2009; 3: 635-43.
Search in Google Scholar Back to article
21. Pierucci-Lagha A, Gelernter J, Feinn R, Cubells JF, Pearson D, Pollastri A, et al. Diagnostic reliability of the Semi-structured Assessment for Drug Dependence and Alcoholism (SSADDA). Drug Alcohol Depend. 2005; 80:303-12.10.1016/j.drugalcdep.2005.04.00515896927
Open DOI Search in Google Scholar Back to article
22. Wu LT, Pilowsky DJ, Schlenger WE. Inhalant abuse and dependence among adolescents in the United States. J Am Acad Child Adolesc Psychiatry. 2004; 43: 1206-14.10.1097/01.chi.0000134491.42807.a3128312115381887
Open DOI Search in Google Scholar Back to article
23. Johnston LD, O’Malley PM, Bachman JG, Schulenberg JE. Monitoring the future national results on adolescent drug use: overview of key findings. Bethesda, MD: National Institute on Drug Abuse. 2009:14-5.10.1037/e560352009-001
Search in Google Scholar Back to article
24. Riegel AC, Zapata A, Shippenberg TS, French ED. The abused inhalant toluene increases dopamine release in the nucleus accumbens by directly stimulating ventral tegmental area neurons. Neuropsychopharmacology. 2007; 32:1558-69.10.1038/sj.npp.130127317213847
Open DOI Search in Google Scholar Back to article
25. Di Chiara G, Bassareo V, Fenu S, De Luca MA, Spina L, Cadoni C, et al. Dopamine and drug addiction: the nucleus accumbens shell connection. Neuropharmacology. 2004; 47:227-41.10.1016/j.neuropharm.2004.06.03215464140
Open DOI Search in Google Scholar Back to article
26. Volkow ND, Fowler JS, Wang GJ, Swanson JM, Telang F. Dopamine in drug abuse and addiction: results of imaging studies and treatment implications. Arch Neurol. 2007; 64:1575-9.10.1001/archneur.64.11.157517998440
Open DOI Search in Google Scholar Back to article
27. Klanker M. Dopamine/Serotonin interactions in reward-related behavior: Focus on the Iowa Gambling Task: Utrecht University. 2008.
Search in Google Scholar Back to article
28. Axelrod J. The metabolism of catecholamines in vivo and in vitro. Pharmacol Rev. 1959; 11:402-8.
Search in Google Scholar Back to article
29. Starr JM, Fox H, Harris SE, Deary IJ, Whalley LJ. COMT genotype and cognitive ability: a longitudinal aging study. Neurosci Lett. 2007; 421:57-61.10.1016/j.neulet.2007.05.02317548151
Open DOI Search in Google Scholar Back to article
30. Egan MF, Goldberg TE, Kolachana BS, Callicott JH, Mazzanti CM, Straub RE, et al. Effect of COMT Val108/158 Met genotype on frontal lobe function and risk for schizophrenia. Proc Natl Acad Sci U S A. 2001; 98:6917-22.10.1073/pnas.1111345983445311381111
Search in Google Scholar Back to article
31. Wu LT, Howard MO, Pilowsky DJ. Substance use disorders among inhalant users: results from the National Epidemiologic Survey on Alcohol and Related Conditions. Addict Behav. 2008; 33:968-73.10.1016/j.addbeh.2008.02.019242284818403132
Open DOI Search in Google Scholar Back to article
32. Kelder SH, Murray NG, Orpinas P, Prokhorov A, McReynolds L, Zhang Q, et al. Depression and substance use in minority middle-school students. Am J Public Health. 2001; 91:761-6.10.2105/AJPH.91.5.761144666811344884
Search in Google Scholar Back to article
33. Kubik MY, Lytle LA, Birnbaum AS, Murray DM, Perry CL. Prevalence and correlates of depressive symptoms in young adolescents. Am J Health Behav. 2003; 27:546-53. 10.5993/AJHB.27.5.614521250
Open DOI Search in Google Scholar Back to article
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Polymorphism of COMT Val158Met is associated with inhalant use and dependence: a Thai substance dependence treatment cohort

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